www.elpisbio.com

 

 

intranet

 

 

 

 

 

 

 

 

HOME

PRODUCTS

SUPPORT

Q & A

USEFUL SITES

About Us

from GENE to PROTEIN

from GENE to PROTEIN

from GENE to PROTEIN

from GENE to PROTEIN

from GENE to PROTEIN

from GENE to PROTEIN

 

 

 

PROTEIN BIOCHEMISTRY

 

MOLECULAR BIOLOGY

 

Prestained Protein Markers

 

DNA Size Ladder Markers

 

Unstained Protein Markers

 

PCR Enzymes

 

Western Markers

 

PCR Buffer & Additives

 

Protein Gel Electrophoresis Kits

 

PCR Master Mix & Premixes

 

Gel Electrophoresis Reagents

 

cDNA Synthesis Enzymes

 

Premade Buffers

 

RT Master Mixes

 

Protein Gel Stains

 

Gene Cloning Kits

 

Protein Purification Resin & Kit

 

Site-specific Mutagenesis Kit

 

Western Detection Reagents

 

Polymerases

 

Site-specific Endoproteases

 

Endo-, Exo-Nucleases

 

Endo-, Exo-glycosidases

 

Ligase/Kinase/Phosphatase

 

Protein Enzymes

 

Recombinases

 

 

 

Restriction Enzymes

 

Human Proteins

 

Nucleic Acid Purification Kits

 

 

 

Reagents & Buffers

 

 

 

Modification Enzymes

 

 

 

 

 Gradi-Gel II Gradient PAGE Analysis Kit

 

Products

Qty.

Cat. No.

Conc.

Price(W)

Protocol

MSDS

 Gradi-Gel II Gradient PAGE Analysis Kit

1 Kit

EBA-1056

50 (29:1)

150,000

 

Description

Gradi-Gel II gradient PAGE analysis kit is a novel product of Elpis Biotech to make up for defects of conventional gradient gel preparation. Preparation of conventional gradient gel using a gradient former is a time consuming and tedious job itself. Furthermore, hand-made gradient gel is not reproducible in electrophoretic results and sometimes results in distorted images because of difference in polyacrylamide gel percentage between upper and lower part.

Gradi-Gel II gradient PAGE analysis kit uses single percentage polyacrylamide gel  employing the chemical gradient formation during gel electrophoresis instead of mechanical gradient. Gradi-Gel II gradient PAGE analysis kit is composed of 2x running gel buffer, 2x stacking gel buffer, and acrylamide stock solution. By simply mixing buffers and acrylamide solution in kit, users can prepare a linear migrating gradient gel without unnecessary labor.

Gradi-Gel II gradient running gel is overlaid with a lower percentage stacking gel, ensuring highly separating performance and provide you with highly resolved razor-sharp protein banding.

Gradi-Gel II gradient PAGE analysis kit is completely compatible with conventional Tris-Glycine-SDS running buffer for denaturing PAGE, and Tris-Glycine for native and non-denaturing PAGE.

1. Convenient, ready-to-use, reproducible gradient polyacrylamide gels

2. High resolution (razor-sharp bands)

3. Fast running speed (1.5-2 hrs at 100 V constant voltage)

4. No need to buffer change (Tris-Glycine-SDS buffer system)

5. Small peptide analysis using low percentage gels

6. Optimized composition for western transfer and blotting, brilliant blue and silver staining of gels

7. No distortion and shrinking of dried gels (because of single % gradient gel)

Storage   Store at 4℃ for 1 year

                                                        

Kit contents

- Gradi-Gel II 2x Stacking Gel Buffer :  20 ml

- Gradi-Gel II 2x Running Gel Buffer : 100 ml

- 30% Acrylamide Stock Solution (acrylamide:bisacrylamide = 29:1) : 100 ml

             

 

    

    

 

Contact Us

PARTNERSHIP

LOCATION

e-CATALOG

All PRODUCTS

CopyrightⓒELPIS-BIOTECH. Inc. All Rights Reserved.  

Contact elpis@elpisbio.com for more Information.