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from GENE to PROTEIN

from GENE to PROTEIN

from GENE to PROTEIN

from GENE to PROTEIN

from GENE to PROTEIN

from GENE to PROTEIN

 

 

 

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 His-Bind Agarose Resin

 

Products

Qty.

Cat. No.

Conc.

Price(W)

Protocol

MSDS

 His-Bind Agarose Resin

10 ml

EBE-1031

75% slurry

80,000

 

Description

His•Bind agarose resin is designed for a rapid purification of recombinant proteins containing His•Tag sequence by IMAC. The His•Tag sequence binds to Ni2+ cations, which are immobilized on the His•Bind agarose resin. After unbound proteins are washed away, the target protein is recovered by elution with imidazole-containing wash buffer. The His•Bind agarose resin can be regenerated and reused several times.

Features

Matrix 

Agarose 4B, cross-linked

Bead Size

45-165 mm

Activation 

Epoxy

Attachment  

Iminodiacetic acid (IDA), Ni ion pre-charged

Linker Size   

12-atom space linker

Binding Capacity  

5-10 mg His•Tagged proteins/ml resin

pH Stability

pH 2 to 14

Storage Buffer 

75% slurry in PBS with 20% ethanol

Storage  Store at 4℃, do not freeze

 

          

 

         

 

 

 

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