www.elpisbio.com

 

 

intranet

 

 

 

 

 

 

 

 

HOME

PRODUCTS

SUPPORT

Q & A

USEFUL SITES

About Us

from GENE to PROTEIN

from GENE to PROTEIN

from GENE to PROTEIN

from GENE to PROTEIN

from GENE to PROTEIN

from GENE to PROTEIN

 

 

 

PROTEIN BIOCHEMISTRY

 

MOLECULAR BIOLOGY

 

Prestained Protein Markers

 

DNA Size Ladder Markers

 

Unstained Protein Markers

 

PCR Enzymes

 

Western Markers

 

PCR Buffer & Additives

 

Protein Gel Electrophoresis Kits

 

PCR Master Mix & Premixes

 

Gel Electrophoresis Reagents

 

cDNA Synthesis Enzymes

 

Premade Buffers

 

RT Master Mixes

 

Protein Gel Stains

 

Gene Cloning Kits

 

Protein Purification Resin & Kit

 

Site-specific Mutagenesis Kit

 

Western Detection Reagents

 

Polymerases

 

Site-specific Endoproteases

 

Endo-, Exo-Nucleases

 

Endo-, Exo-glycosidases

 

Ligase/Kinase/Phosphatase

 

Protein Enzymes

 

Recombinases

 

 

 

Restriction Enzymes

 

Human Proteins

 

Nucleic Acid Purification Kits

 

 

 

Reagents & Buffers

 

 

 

Modification Enzymes

 

 

 

 

 His-Bind Spin Column

 

Products

Qty.

Cat. No.

Conc.

Price(W)

Protocol

MSDS

 His-Bind Spin Columns

20 ea

EBE-1033

 

120,000

 

Description

His•Bind Spin Column allow a rapid procedure for purification of polyhistidine-tagged proteins from crude cell extracts. Beginning with the clarified cell extracts, the entire procedure to purify polyhistidine-tagged proteins takes approximately 5 minutes. Highly purified proteins can be obtained with simple loading, washing, and elution by centrifugation. The spin column contains a resin which cross-linked with iminodiacetic acid as a ligand, pre-packed and pre-charged with Ni+ ion. The spin columns are highly specific for polyhistidine-tagged proteins and exhibits very low non-specific binding of other proteins. The spin columns are reusable at least three times by serial washing with elution buffer and binding buffer without prominent loss of binding efficiency.

Features

Attachment  

Iminodiacetic acid (IDA), Ni ion pre-charged

Loading Volume   

< 700 ml

Elution Volume

 > 50 ml

Binding Capacity  

400 mg (~25kDa)

Storage  Store at 4℃, do not freeze

 

          

 

      

 

 

 

Contact Us

PARTNERSHIP

LOCATION

e-CATALOG

All PRODUCTS

CopyrightⓒELPIS-BIOTECH. Inc. All Rights Reserved.  

Contact elpis@elpisbio.com for more Information.