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 PRO-Hunt His-Bind Purification Kit (spin type)

 

Products

Qty.

Cat. No.

Conc.

Price(W)

Protocol

MSDS

 PRO-Hunt His-Bind Purification Kit

1 kit

EBE-1036

20 prep, spin-type

160,000

 

Description

PRO·Hunt His•Bind Purification Kit (spin-type) allow a rapid procedure for the purification of polyhistidine-tagged proteins from crude cell extracts. Beginning with the clarified cell extracts, the entire procedure to purify polyhistidine-tagged proteins takes approximately 5 minutes. Highly purified proteins can be obtained with simple loading, washing, and elution by centrifugation. The spin column contains a resin which cross-linked with iminodiacetic acid as a ligand, pre-packed and pre-charged with Ni2+ ion. The spin columns are highly specific for polyhistidine-tagged proteins and exhibits very low non-specific binding of other proteins. The spin columns are reusable at least three times by serial washing with elution buffer and binding buffer without prominent loss of binding efficiency.

The kit can be used for denaturing (containing chaotropic agent such as urea or guanidine HCl) condition, as well.

The kit contains sufficient amount of Binding Buffer (cell lysis/bind buffer), Washing Buffer, and Elution Buffer as concentrated form for approximately 20 purifications.

Features

- Loading Volume  < 700 ml clarified extracts

- Elution Volume  > 50 ml clarified extracts

- Binding Capacity   < 400 mg  (~25 kDa)

- Ligand   Ni-iminodiacetic acid  (Ni-IDA)

Kit Component

- Spin Columns   20 columns (pre-charged with Ni2+ ion)  and collection tubes

- 5x Binding Buffer   30 ml

- 5x Washing Buffer  30 ml

- 1x Elution Buffer   30 ml

- Control Extract (25 kDa)  1 ml

- Protocol  1 ea

Storage

Store at 4℃, do not freeze

 

        

 

   

     

 

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