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 PRO-Hunt His-Bind Buffer Kit 100

 

Products

Qty.

Cat. No.

Conc.

Price(W)

Protocol

MSDS

 PRO-Hunt His-Bind Buffer Kit 100

1 Kit

EBE-1040

 

80,000

 

Description

PRO·Hunt His•Bind Buffer Kit contains all  buffer components necessary for the purification of polyhistidine-tagged proteins. The buffer kit  provides a convenient set of buffers optimized for a purification of polyhistidine-tagged fusion proteins.

The solutions in the kit can be used just after dilution with 4 part of distilled water.

Kit Component

- 5x Binding Buffer   100 ml  (1x ; 0.5 M NaCl, 5 mM imidazole, 20 mM Tris-HCl, pH 7.9)

- 5x Washing Buffer  100 ml  (1x ; 0.5 M NaCl, 60 mM imidazole, 20 mM Tris-HCl, pH 7.9)

- 1x Elution Buffer   100 ml  (1x ; 0.25 M NaCl, 0.5 M imidazole, 10 mM Tris-HCl, pH 7.9)

- 5x Charging Buffer (NiSO4)  50 ml  (1x ; 50 mM NiSO4)

Storage

Store at 4℃, do not freeze

 

      

 

 

 

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