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 HRV 3C Protease, 6xHis

 

Products

Qty.

Cat. No.

Conc.

Price(W)

Protocol

MSDS

 HRV 3C Protease, 6xHis

2,000 unit

EBF-1001

20 unit/ml

250,000

10,000 unit

EBF-1002

20 unit/ml

800,000

 

Description

HRV 3C protease is a site-specific endoprotease that cleaves after glutamine at its cleavage site Leu-Glu-Val-Leu-Phe-Gln-/-Gly-Pro, commonly referred to as the PreScission Site. It cleaves between Gln and Gly. HRV 3C protease is a cysteine protease comes from Human Rhinovirus. 

Recombinant HRV 3C protease is purified from E.coli containing a clone of the Human Rhinovirus – HRV 3C protease gene. Since HRV 3C protease contains 6x His tag (cat# : EBF-1001 and 1002) or 6x His-GST tag (cat# : EBF-1003 and 1004) sequence, they can be easily removed by a metal affinity or glutathione (GSH) affinity chromatography after the cleavage reaction. The recombinant 6x His tagged HRV 3C protease is 22 kDa, and 6x His-GST tagged HRV 3C protease is 48.5 kDa in size.

Substrate Specificity

-Leu-Glu-Val-Leu-Phe-Gln - / - Gly-Pro-

Product Component

- HRV 3C Protease : 100 μl (100 mg, 2,000 units), or 500 μl (500 mg, 10,000 units)

- Control protein : 30 mg (1 mg/ml), produce 43.8 kDa and 48.4 kDa by HRV 3C protease

Unit Definition

One mg of HRV 3C protease has an equivalent to 10-20 unit activity. The activity of HRV 3C protease is tested using a control

substrate protein. One unit will cleave >90% of 100 μg of protein in standard buffer at 4°C in 16 hrs.

Specific Activity

HRV 3C Protease (6x His Tag) : 20 unit/mg protein

HRV 3C Protease (6x His, GST Tag) : 10 unit/mg protein

Storage Buffer

50 mM Tris-HCl, pH 8.0, 150 mM NaCl, 1 mM EDTA, 0.5 mM DTT, and 50% glycerol.

HRV 3C Reaction Condition

20 mM Tris-HCl, pH 7.0, 150 mM NaCl, 1 mM DTT, 0.5 mM EDTA (optional)

caution : we do not provide reaction buffer in the product

Storage Temperature

Store at -20℃

QC Tests: 

Activity, SDS-PAGE purity, performance tests

 

 

 

         

 

 

 

 

       

 

 

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