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 SenP2 SUMO Protease

 

Products

Qty.

Cat. No.

Conc.

Price(W)

Protocol

MSDS

 SenP2 SUMO Protease, 6xHis

500 unit

EBF-1032

10 unit/ml

250,000

 

Description

SENP2 peptidase is one of SUMO-specific protease (SENP) family in human, and involved in the SUMO conjugation through the processing of SUMO1, SUMO2, and SUMO3 to mature form and deconjugation of SUMOs from targeted proteins. SENP2 has known to down-regulate CTNNB1 levels and thereby modulate the Wnt pathway Like Ulp1, SENP2 hydrolysis the peptide bond in the sequence Gly-Gly-!Ala-Thr-Tyr at the C-terminal end of SUMOs. The catalytic domain  (363-588 aa) of SENP2 is capable of removal of SUMO tag from recombinant fusion protein.

SENP2 (363-588 aa) was overexpressed and purified from E.coli strain as His-tagged form.

 - Removal of SUMO tag from recombinant fusion protein.

 - Highly specific  cleavage.

 - Broad range of active temperature (4°C ~ 37°C).

Concentration & Storage Condition

 10 unit/ml.  Store at -20℃.

Storage Buffer

 50 mM Tris-HCl, pH 7.5, 150 mM NaCl,  1 mM DTT, 50% glycerol.

Reaction condition

 50 mM Tris-HCl, pH 7.5, 100 mM NaCl,  1 mM DTT, 10% glycerol.

Unit Definition

 One unit is defined as the amount of enzyme required to cleave > 85% of 100 mg of sumo-fusion protein at 37°C for 1 hour or 4°C for overnight.

QC Tests

 Activity test, SDS-PAGE purity. 

 

 

 

 

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