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 pLPS-T TA Topo Vector

 

Products

Qty.

Cat. No.

Conc.

Price(W)

Protocol

Brochure

 pLPS-T TA Topo Vector

25 rxn

EBK-1005

1 rxn/ml

200,000

250 rxn

EBF-1006

1 rxn/ml

1,500,000

 

Description

The pLPS-T TA topo vector is a high efficient and very convenient tool for a direct cloning of PCR products. The type I topoisomerase from Vaccinia virus can cut and rejoin the DNA fragment without the action of DNA ligase. Topoisomerase I premixed in the vector solution rejoins the input DNA fragments to vector ends within 5 min at 37℃. 

There is no need to consider whether insert DNA has been phosphorylated. Furthermore, there is no need to use IPTG/X-gal in the transformation process due to its high ligation and cloning efficiency (> 90%). This means you can obtain 9 real clones from 10  randomly picked colonies.

The pLPS-T TA vector is suitable for the cloning of A tailed DNA fragments, generally produced by Taq polymerases, like rTaq, rTaq plus, and some blend Taqs. If your DNA has no 3'-A tails, you should add A tails by appropriate methods before start.

By using pLPS-T TA vector system, up to 5 kbp sized DNA fragments can be easily cloned without the remarkable loss of efficiency. 

The pLPS-T TA vector is provided in 50% buffered glycerol containing red dye to easily visualize vector solution. Simply mix 1 μl of vector solution with 1 μl of 5x reaction buffer and your DNA to final 5 μl, and incubate for 5 min at 37℃. Then you are already ready to transform E.coli. In the next day, you can get the clone in plasmid.

Kit Component : EBK-1005

   25 μl pLPS-T TA Vector

   50 μl 5x Topo Reaction Buffer

   5 μl Control Insert DNA (A-tailed) 

Kit Component : EBK-1006

   250 μl pLPS-T TA Vector

   500 μl 5x Topo Reaction Buffer

   5 μl Control Insert DNA (A-tailed)  

QC tests

Performance tests.

Storage Condition

Store at -20℃.

 

Support

  - Vector Sequence

 

 

 

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