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 Overlap Cloner DNA Cloning Kit

 

Products

Qty.

Cat. No.

Conc.

Price(W)

Protocol

Brochure

 Overlap Cloner DNA Cloning Kit

25 rxn

EBK-1011

1 rxn/ml

200,000

NEWS LETTER

100 rxn

EBF-1012

1 rxn/ml

600,000

 

Description

 

The Overlap Cloner DNA Cloning Kit provides simple and fast directional cloning of one or more DNA fragments into any vectors. The most advantages of this system are that the insert DNA can be precisely cloned as intended without additional bases and several fragments can be connected with intentional direction at one time reaction. This system requires no site-specific recombination sites or extra DNA sequences and eliminates the needs for restriction enzymatic and DNA ligase treatments. The enzyme mix provided with this system recognizes a more than 15 bp overlap at each ends and precisely connects the DNA fragments sharing this homology. This 15 bp overlap can be generated by PCR amplification with designing primers. This system can be used for up to 4 fragments cloning into vector, totaling up to 10 kb in length.

  The Overlap Cloner DNA Cloning Kit offers increased cloning efficiency over classical cloning methods using restriction enzymes and T4 DNA ligase, especially for long fragments and multiple fragments along with no additional bases.

 

                   

 

Applications :

1. Simple cloning (ligation independent)

2. Easy subcloning to destination vectors

3. Highly efficient library construction

4. Gene fusion (advanced than overlapping PCR) 

5. No additional sequences in expression study

6. Vector reconstitution study in one tube

7. One pot reaction of in vitro gene synthesis

Advantages :

1. No use of restriction enzymes & ligase

2. Rapid reaction time : 30~60 min reaction

3. High efficiency : up to 90% when single insert used

4. Multiple ligation : up to 6 DNA fragments in one reaction

5. Exact in-frame cloning : no additional bases or amino acids

 

     

 

Kit Components

 

         Components                                  25 Reactions               100 Reactions                  Storage

                                                                       (EBK-1011)                  (EBK-1012)                                     

   Overlap Cloner Enzyme Mix                            25 ml                              100 ml                           -20℃

   Overlap Cloner 10x Reaction Buffer             100 ml                              500 ml                           -20℃

   Control Vector (50 ng/ml)                                    5 ml                                 25 ml                           -20℃

   Control Insert (LacZ DNA, 50 ng/ml)                  5 ml                                 25 ml                           -20℃

   Manual                                                                1 ea                                1 ea                                -      

 

 

Action Mechanism :

 

                 

 

 

Optimal Reaction Condition :

 

 

 

 

Exp : Multiple Fragment Ligation :

   

 

 

Storage Condition

The Overlap Cloner DNA Cloning Kit should be stored at  -20℃ or below. The kit can be stored for up to 12 months without showing any reduction in performance and quality.

 

 

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