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from GENE to PROTEIN

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PROTEIN BIOCHEMISTRY

 

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 Mid-range Pre-stained Marker (dual-color)

 

Products

Qty.

Cat. No.

Conc.

Price(W)

Protocol

MSDS

 Mid-range Pre-stained Marker  (dual-color)

500 ml

EBM-1018

100

100,000

 

Description

Elpis Biotech’s mid-range pre-stained protein size markers are mixture of 7 recombinant purified and pre-stained polypeptides whose molecular weights are well-adjusted. The each pre-stained protein size marker is available in a ready-to-use formula (no need to boil before use), and is suitable for sizing proteins between 15,000 and 100,000 Da by SDS-PAGE. Chromophores are covalently bound to proteins, and 7 pre-stained proteins are visible during electrophoresis or electrophoretic transfer from the gel to the membrane. The 50 kDa protein added double enables easy identification of migrating bands.

Because the coupling of chromophore to the proteins affects their apparent molecular weights in SDS-PAGE relative to un-stained proteins, un-stained protein standards should be used for accuracy >95%.

Recommended loading volume

   5 ml/lane for a mini-gel (10 x 8㎠, 0.75㎜ or 1㎜ thick)

  10-15 ml/lane for a large-gel (30 x 20㎠, 1㎜ or 1.5㎜ thick)

Recommended storage condition

  -20℃ for one year

   Please aliquot in a small volume for prevention of  repeated freezing & thawing

 

 

   

     

 

 

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