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from GENE to PROTEIN |
from GENE to PROTEIN |
from GENE to PROTEIN |
from GENE to PROTEIN |
from GENE to PROTEIN |
from GENE to PROTEIN |
PROTEIN BIOCHEMISTRY |
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MOLECULAR BIOLOGY |
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Broad-range Pre-stained Marker (dual-color)
Products |
Qty. |
Cat. No. |
Conc. |
Price(W) |
Protocol |
MSDS |
Broad-range Pre-stained Marker (dual color) |
500 ml |
EBM-1032 |
100 |
160,000 |
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Broad-range Protein Markers are useful for SDS-PAGE analysis of your proteins. The each marker is a mixture of 10 recombinant purified proteins whose molecular weights are well-defined. The markers are supplied in a ready-to-use formula (no need to boil before use), and is suitable for sizing proteins between 7,000 and 240,000 Da by SDS-PAGE.
Chromophores are covalently linked to proteins in pre-stained markers, and 10 pre-stained proteins are visible during electrophoresis or electrophoretic transfer from the gel to the membrane. The 25 and 70 kDa proteins play a ruler function for easy identification of banding.
Because coupling of chromophores to the proteins in pre-stained markers affects their apparent molecular weights in SDS-PAGE relative to un-stained proteins, un-stained protein standard (Cat. No. EBM-1034) should be used for accuracy >95%.
- 5 ml/lane for a mini-gel (10x8 cm2, 0.75 mm or 1 mm thick) - 10-15 ml/lane for a large-gel (30x20 cm2, 1 mm or 1.5 mm thick) - 2-5 ml/lane for a western transfer onto PVDF or NC membranes
- Store at -20oC for one year - Please aliquot in a small volume for prevention of repeated freezing & thawing
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Contact elpis@elpisbio.com for more Information.