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from GENE to PROTEIN

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PROTEIN BIOCHEMISTRY

 

MOLECULAR BIOLOGY

 

Prestained Protein Markers

 

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 Broad-range Pre-stained Marker (dual-color)

 

Products

Qty.

Cat. No.

Conc.

Price(W)

Protocol

MSDS

 Broad-range Pre-stained Marker  (dual color)

500 ml

EBM-1032

100

160,000

 

Description

Broad-range Protein Markers are useful for SDS-PAGE analysis of your proteins. The each marker is a mixture of 10 recombinant purified proteins whose molecular weights are well-defined. The markers are supplied in a ready-to-use formula (no need to boil before use), and is suitable for sizing proteins between 7,000 and 240,000 Da by SDS-PAGE.

Chromophores are covalently linked to proteins in pre-stained markers, and 10 pre-stained proteins are visible during electrophoresis or electrophoretic transfer from the gel to the membrane. The 25 and 70 kDa proteins play a ruler function for easy identification of banding.

Because coupling of chromophores to the proteins in pre-stained markers affects their apparent molecular weights in SDS-PAGE relative to un-stained proteins, un-stained protein standard (Cat. No. EBM-1034) should be used for accuracy >95%.

Recommended loading volume

  -  5 ml/lane for a mini-gel (10x8 cm2, 0.75 mm or 1 mm thick)

  - 10-15 ml/lane for a large-gel (30x20 cm2, 1 mm or 1.5 mm thick)

  -  2-5 ml/lane for a western transfer onto PVDF or NC membranes

Recommended storage condition

  - Store at -20oC for one year

  - Please aliquot in a small volume for prevention of repeated freezing & thawing

 

  

 

      

      

 

 

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