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from GENE to PROTEIN

from GENE to PROTEIN

from GENE to PROTEIN

from GENE to PROTEIN

from GENE to PROTEIN

from GENE to PROTEIN

 

 

 

PROTEIN BIOCHEMISTRY

 

MOLECULAR BIOLOGY

 

Prestained Protein Markers

 

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 Color Plus Protein Marker

 

Products

Qty.

Cat. No.

Conc.

Price(W)

Protocol

MSDS

 Color Plus Protein Marker

500 ml

EBM-1035

100

160,000

 

Description

Color Plus Pre-stained protein marker is useful for SDS-PAGE analysis of your proteins. The each marker is a mixture of 10 recombinant purified polypeptides whose molecular weights are well-confined. The marker is supplied in a ready-to-use formula (no need to boil before use), and is suitable for sizing proteins between 7,000 and 200,000 Da by SDS-PAGE.

Chromophores are covalently linked to proteins in pre-stained markers, and 10 pre-stained proteins are visible during electrophoresis or electrophoretic transfer from the gel to a membrane. The 72 kDa protein play a ruler function for easy identification of banding.

Because coupling of chromophore to the proteins in pre-stained markers affects their apparent molecular weights in SDS-PAGE relative to un-stained proteins, un-stained protein standard should be used for accuracy >95%.

Recommended loading volume

    5 ㎕/lane for a mini-gel (10x8 ㎠, 0.75 ㎜ or 1 ㎜ thick)

  ◈ 10-15 ㎕/lane for a large-gel (30x20 ㎠, 1 ㎜ or 1.5 ㎜ thick)

    2 ㎕/lane for a western transfer onto PVDF or NC membranes

Recommended storage condition

  ◈ -20℃ for one year

    Please aliquot in a small volume for prevention of repeated freezing & thawing

 

    

 

 

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