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from GENE to PROTEIN

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PROTEIN BIOCHEMISTRY

 

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 EPD Western Reagent

 

Products

Qty.

Cat. No.

Conc.

Price(W)

Protocol

MSDS

 EPD Western Reagent

1 kit

EBP-1071

200 ml

80,000

 

Description

EPD (Enhanced Peroxidase Detection) western Reagent Kit consists of enhanced chemiluminescent substrate for horseradish peroxidase (HRP) on immunoblots.

Kit contents

1. EPD Substrate   Solution A 100 ml  and Solution B 100 ml.

2. Rapid protocol

Storage Condition

Store at 2-8°C, and then stable for at least 6 months.

Additional reagents required

Phosphate-buffered saline (PBS) pH7.5 ; 80 mM Na2HPO4, 20 mM NaH2PO4, 100 mM NaCl

Tris-buffered saline (TBS) pH7.5 ; 20 mM Tris-HCl, 137 mM NaCl

Tween-20

PBS-T or TBS-T ; 0.05% to 1% of Tween-20 in PBS or TBS

Blocking reagents (BSA or non-fat dried milk) ; usually 1 – 5% in PBS-T or TBS-T 

Primary antibody and HRP-secondary antibody ; It is recommended that the antibody dilution   should be optimized empirically to maximize signal to noise

Critical parameters

1. Proper dilution of primary and secondary antibodies for the best results.

2. Proper blocking and washing to minimize nonspecific signals.

 

   

 

 

 

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