www.elpisbio.com

 

 

intranet

 

 

 

 

 

 

 

 

HOME

PRODUCTS

SUPPORT

Q & A

USEFUL SITES

About Us

from GENE to PROTEIN

from GENE to PROTEIN

from GENE to PROTEIN

from GENE to PROTEIN

from GENE to PROTEIN

from GENE to PROTEIN

 

 

 

PROTEIN BIOCHEMISTRY

 

MOLECULAR BIOLOGY

 

Prestained Protein Markers

 

DNA Size Ladder Markers

 

Unstained Protein Markers

 

PCR Enzymes

 

Western Markers

 

PCR Buffer & Additives

 

Protein Gel Electrophoresis Kits

 

PCR Master Mix & Premixes

 

Gel Electrophoresis Reagents

 

cDNA Synthesis Enzymes

 

Premade Buffers

 

RT Master Mixes

 

Protein Gel Stains

 

Gene Cloning Kits

 

Protein Purification Resin & Kit

 

Site-specific Mutagenesis Kit

 

Western Detection Reagents

 

Polymerases

 

Site-specific Endoproteases

 

Endo-, Exo-Nucleases

 

Endo-, Exo-glycosidases

 

Ligase/Kinase/Phosphatase

 

Protein Enzymes

 

Recombinases

 

 

 

Restriction Enzymes

 

Human Proteins

 

Nucleic Acid Purification Kits

 

 

 

Reagents & Buffers

 

 

 

Modification Enzymes

 

 

 

 

 PicoEPD Western Reagent

 

Products

Qty.

Cat. No.

Conc.

Price(W)

Protocol

MSDS

 PicoEPD Western Reagent

1 kit

EBP-1073

200 ml

100,000

 

Description

PicoEPD (Enhanced Peroxidase Detection) Western Reagent Kit consists of a highly sensitive and stable chemiluminescent substrate for horseradish peroxidase (HRP) on immunoblots. The extremely intense signal output and stable emission of blue light for relatively long time (<12 hr) of PicoEPD substrate enables the detection of picogram quantities of antigen in immunoblot and direct or indirect South/Northern blot using HRP-conjugated polynucleotide probe.

Kit contents

1. PicoEPD Substrate   Solution A 100 ml  and Solution B 100 ml.

2. Rapid protocol

Storage Condition

Store at 2-8°C, and then stable for at least 6 months.

Additional reagents required

Phosphate-buffered saline (PBS) pH7.5 ; 80 mM Na2HPO4, 20 mM NaH2PO4, 100 mM NaCl

Tris-buffered saline (TBS) pH7.5 ; 20 mM Tris-HCl, 137 mM NaCl

Tween-20

PBS-T or TBS-T ; 0.05% to 1% of Tween-20 in PBS or TBS

Blocking reagents (BSA or non-fat dried milk) ; usually 1 – 5% in PBS-T or TBS-T 

Primary antibody and HRP-secondary antibody ; It is recommended that the antibody dilution   should be optimized empirically to maximize signal to noise

Critical parameters

1. Proper dilution of primary and secondary antibodies for the best results.

2. Proper blocking and washing to minimize nonspecific signals.

 

   

 

   

   

 

 

 

Contact Us

PARTNERSHIP

LOCATION

e-CATALOG

All PRODUCTS

CopyrightⓒELPIS-BIOTECH. Inc. All Rights Reserved.  

Contact elpis@elpisbio.com for more Information.