Aat II

 

Products	Qty.	Cat. No.	Conc.	Price(W)	Protocol	MSDS
Aat II            GACGT^C	250 unit	EBR-1001	5 unit/ml	80,000
Source   Acetobacter aceti. Reaction Conditions   Buffer 4.  20 mM Tris-acetate, pH7.9, 50 mM potassium acetate,   10 mM magnesium acetate, 1 mM DTT. Incubate at 37℃. Concentration & Storage Condition   Store at -20℃. 5 unit/ml in 10 mM Tris-HCl, pH 7.4, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 200 mg/ml BSA, 50% (v/v) glycerol. Heat Inactivation Condition   65℃ for 20 min. Enzyme Dilution   Dilute with Storage Buffer. QC Tests   Activity, ligation and recutting, exo and endonuclease activity test, SDS-PAGE purity, performance tests. Methylase Sensitivity   Blocked by CpG methylation (Cleavage of mammalian genomic DNA is blocked).   Usage Guidelines   - Restriction enzyme double digestion guide   - Restriction enzyme buffer activiy chart   - Restriction enzyme dilution buffer table   - Restriction enzyme all product table