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 HiPi DNA Polymerase

 

Products

Qty.

Cat. No.

Conc.

Price(W)

Protocol

MSDS

 HiPi DNA Polymerase

500 units

EBT-1002

5 unit/ml

100,000

 

Description

HiPi DNA Polymerase is a purified recombinant thermostable DNA polymerase from Thermus aquaticus. This enzyme is modified to increase specificity, fidelity, and performance in conventional PCR process. HiPi DNA Polymerase is recommended for use in conventional PCR that final products are  less than 3 kbp . HiPi DNA Polymerase generates a 3´-dA overhang suitable for a TA cloning of PCR products.

Storage Buffer

5 unit/ml in 20 mM Tris-HCl, pH8.0, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5% Tween 20, and 50% Glycerol.

Applications

PCR, RT-PCR, genomic PCR, and primer extension.

Unit Definition

One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmole of dNTP into an acid-insoluble form in 30 min at 72℃. The reaction conditions are : 25 mM TAPS, pH9.3, 50 mM KCl, 2 mM MgCl2, 1 mM b-mercaptoethanol, 200 mM each dNTPs, 100 mM [a-32P]dCTP, 12.5 mg activated calf thymus DNA in a total volume of 50 ml.

10x PCR Reaction Buffer w/ MgCl2

500 mM Tris-HCl, pH9.0, 160 mM (NH4)2SO4, 25 mM MgCl2, 1% Tween 20, 1 mg/ml BSA.

QC tests

Activity, SDS-PAGE purity, performance tests.

Storage Condition

Store at -20℃.

 

 

   

 

 

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