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 HiPi Plus DNA Polymerase

 

Products

Qty.

Cat. No.

Conc.

Price(W)

Protocol

MSDS

 Hipi Plus DNA Polymerase

500 units

EBT-1007

5 unit/ml

120,000

 

Description

HiPi Plus DNA Polymerase is suitable for a high fidelity amplification of DNA fragments. This enzyme is designed for a reliable amplification of long (up to 10 kbp for lambda DNA) and complex targets with a robust yield and a high specificity. HiPi Plus DNA Polymerase generates a mixture of PCR products with blunt end and 3´-dA overhangs.

Storage Buffer

5 unit/ml in 20 mM Tris-HCl, pH8.0, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5% Tween 20, and 50% Glycerol.

Applications

High fidelity PCR for a cloning

RT-PCR

Genomic PCR

Long PCR

Highly specific amplification of DNA fragments with high GC content

Unit Definition

One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmole of dNTP into an acid-insoluble form in 30 min at 72℃. The reaction conditions are : 25 mM TAPS, pH9.3, 50 mM KCl, 2 mM MgCl2, 1 mM b-mercaptoethanol, 200 mM each dNTPs, 100 mM [a-32P]dCTP, 12.5 mg activated calf thymus DNA in a total volume of 50 ml.

10x PCR Reaction Buffer w/ MgCl2

500 mM Tris-HCl, pH9.0, 160 mM (NH4)2SO4, 35 mM MgCl2, 1% Tween 20, 1 mg/ml BSA.

10x Q Buffer

PCR additive for increasing specificity of PCR amplification (see figures). Q buffer can be added as 0.5x or 1x to PCR mix.

 

 

 

   

 

 

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