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from GENE to PROTEIN |
from GENE to PROTEIN |
from GENE to PROTEIN |
from GENE to PROTEIN |
from GENE to PROTEIN |
from GENE to PROTEIN |
PROTEIN BIOCHEMISTRY |
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MOLECULAR BIOLOGY |
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T4 DNA Polymerase
Products |
Qty. |
Cat. No. |
Conc. |
Price(W) |
Protocol |
MSDS |
T4 DNA Polymerase |
200 unit |
EBT-1023 |
5 unit/ml |
120,000 |
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T4 DNA Polymerase catalyzes the 5' to 3´ synthesis of DNA from a primed single-stranded DNA template. Although possessing a potent 3' to 5´ proof-reading exonuclease, T4 DNA Polymerase contains no 5' to 3´ exonuclease activity. T4 DNA Polymerase can be used to fill 5´ protruding ends with labeled or unlabeled dNTPs, or for the generation of blunt ends from DNA molecules with 3´ overhangs. T4 DNA Polymerase is purified from a recombinant E.coli strain.
5 unit/ml. Store at -20℃.
200 mM potassium phosphate, pH 6.5, 2 mM DTT and 50% glycerol.
250 mM Tris-Acetate, pH 7.7, 1 M potassium acetate and 10 mM DTT.
One unit is defined as the amount of enzyme required to catalyze the incorporation of 55 nmole of dTTP into acid-precipitable material in 30 min at 37°C using poly(dA):oligo(dT) as a substrate.
Activity, exo and endonuclease activity test, SDS-PAGE purity, performance tests.
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