T4 DNA Polymerase

 

Products	Qty.	Cat. No.	Conc.	Price(W)	Protocol	MSDS
T4 DNA Polymerase	200 unit	EBT-1023	5 unit/ml	120,000
Description T4 DNA Polymerase catalyzes the 5' to 3´ synthesis of DNA from a primed single-stranded DNA template. Although possessing a potent 3' to 5´ proof-reading exonuclease, T4 DNA Polymerase contains no 5' to 3´ exonuclease activity. T4 DNA Polymerase can be used to fill 5´ protruding ends with labeled or unlabeled dNTPs, or for the generation of blunt ends from DNA molecules with 3´ overhangs. T4 DNA Polymerase is purified from a recombinant E.coli strain. Concentration & Storage Condition 5 unit/ml.  Store at -20℃. Storage Buffer   200 mM potassium phosphate, pH 6.5, 2 mM DTT and 50% glycerol. 10x Reaction Buffer 250 mM Tris-Acetate, pH 7.7, 1 M potassium acetate and 10 mM DTT. Unit Definition One unit is defined as the amount of enzyme required to catalyze the incorporation of 55 nmole of dTTP into acid-precipitable material in 30 min at 37°C using poly(dA):oligo(dT) as a substrate. QC Tests   Activity, exo and endonuclease activity test, SDS-PAGE purity, performance tests.