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 One-Step 5x RT-PCR Premix

 

Products

Qty.

Cat. No.

Conc.

Price(W)

Protocol

MSDS

 One-Step 5x RT-PCR Premix

96 Tubes (20 ml)

EBT-1221

96 rxn/20ml

200,000

 

Description

One-step 5x RT-PCR Premix is a mixture of HiPi thermostable DNA polymerase, M-MLV reverse transcriptase, dNTPs, reaction buffer, and stabilizer. Users can perform RT-PCR by simply adding total RNA or poly(A) RNA as substrate and specific primer pair to the premix. HiPi One-step PCR Premix also contains glycerol and bromophenol blue as a tracking dye, enabling a direct analysis of PCR products on a analytic gel electrophoresis. Stabilizer in premix enables prolonging a shelf life at -20℃. You do not need to dissolve the mixture prior to RT-PCR reaction as does competitor’ freeze-dried one. By using HiPi One-step 5x RT-PCR Premix, users can obtain RT-PCR results in a single cycling process. The composition is optimized to reduce non-specific amplification and to minimize primer-dimer formation.

Storage Buffer

1 unit/4 ml thermostable DNA polymerase, 50 units/4 ml M-MLV-RT in 50 mM Tris-HCl, pH8.3, 250 mM KCl, 10 mM MgCl2, 0.05% bromophenol blue, 12% glycerol, and stabilizer.

Applications

One-step RT-PCR.

QC tests

Performance tests.

Storage Condition

Store at -20℃ for one year.

 

  

 

  

 

 

 

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