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 HiPi Super DNA Polymerase

 

Products

Qty.

Cat. No.

Conc.

Price(W)

Protocol

MSDS

 Hipi Super DNA Polymerase

250 units

EBT-1301

5 unit/ml

150,000

500 units

EBT-1302

5 unit/ml

260,000

 

Description

HiPi Super DNA Polymerase is suitable for an amplification of < 40 kbp DNA fragments with a high fidelity and specificity. It is highly effective in long PCR and multiplex PCR.

HiPi Super DNA Polymerase catalyzes the incorporation of nucleotides into duplex DNA in the 5' to 3´ direction in the presence of Mg2+ at 70-80°C. HiPi Super DNA Polymerase is a mix of DNA polymerase showing 3' to 5´ exonuclease (proof-reading) activity and Taq DNA Polymerase. Due to its high elongation rate, long sized products can be generated within relatively shorter running time compared to any other commercially available enzymes (10 kbp within 30 sec extension).

This enzyme is designed for a reliable amplification of long (up to 40 kbp for lambda DNA and 20 kbp for human genomic DNA) and complex targets with a robust yield and a high accuracy. HiPi Super DNA Polymerase generates a mixture of PCR products with blunt end and 3´-dA overhangs.

Applications

PCR for a cloning with  reduced error

RT-PCR

Genomic DNA PCR

Long PCR

Highly specific amplification of DNA fragments with high GC content

Multiplex PCR

Storage Buffer

5 unit/ml in 20 mM Tris-HCl, pH8.0, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5% Tween 20, and 50% Glycerol.

Unit Definition

One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmole of dNTP into an acid-insoluble form in 30 min at 72℃. The reaction conditions are : 25 mM TAPS, pH9.3, 50 mM KCl, 2 mM MgCl2, 1 mM b-mercaptoethanol, 200 mM each dNTPs, 100 mM [a-32P]dCTP, 12.5 mg activated calf thymus DNA in a total volume of 50 ml.

10x Reaction Buffer

500 mM Tris-HCl. pH9.0, 160 mM (NH4)2SO4, 35 mM MgCl2, 1% Triton X-100, 1 mg/ml BSA.

10x Q Buffer

PCR additive for increasing specificity of PCR amplification. Q buffer can be added as 0.5x or 1x to PCR mix.

QC tests

Activity, SDS-PAGE purity, performance tests, genomic DNA contamination test, confirmation test for the absence of endo and exonucleases.

Storage Condition

Store at -20℃.

 

   

 

 

 

 

 

 

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