www.elpisbio.com

 

 

intranet

 

 

 

 

 

 

 

 

HOME

PRODUCTS

SUPPORT

Q & A

USEFUL SITES

About Us

from GENE to PROTEIN

from GENE to PROTEIN

from GENE to PROTEIN

from GENE to PROTEIN

from GENE to PROTEIN

from GENE to PROTEIN

 

 

 

PROTEIN BIOCHEMISTRY

 

MOLECULAR BIOLOGY

 

Prestained Protein Markers

 

DNA Size Ladder Markers

 

Unstained Protein Markers

 

PCR Enzymes

 

Western Markers

 

PCR Buffer & Additives

 

Protein Gel Electrophoresis Kits

 

PCR Master Mix & Premixes

 

Gel Electrophoresis Reagents

 

cDNA Synthesis Enzymes

 

Premade Buffers

 

RT Master Mixes

 

Protein Gel Stains

 

Gene Cloning Kits

 

Protein Purification Resin & Kit

 

Site-specific Mutagenesis Kit

 

Western Detection Reagents

 

Polymerases

 

Site-specific Endoproteases

 

Endo-, Exo-Nucleases

 

Endo-, Exo-glycosidases

 

Ligase/Kinase/Phosphatase

 

Protein Enzymes

 

Recombinases

 

 

 

Restriction Enzymes

 

Human Proteins

 

Nucleic Acid Purification Kits

 

 

 

Reagents & Buffers

 

 

 

Modification Enzymes

 

 

 

 

 rTaq Plus DNA Polymerase

 

Products

Qty.

Cat. No.

Conc.

Price(W)

Protocol

MSDS

 rTaq Plus DNA Polymerase

250 units

EBT-1317

5 unit/ml

60,000

500 units

EBT-1318

5 unit/ml

120,000

 

Description

rTaq Plus DNA Polymerase is a thermostable DNA polymerase that designed for a reliable amplification of long and complex targets with a robust yield in an extremely short extension time. Due to its high elongation rate (about 100 bp/sec) and high processivity (about 250 bases), long sized products can be generated within relatively shorter running time compared to any other commercially available enzymes (10 kbp within 60 sec extension).

Recombinant rTaq Plus DNA Polymerase is purified from a modified recombinant E.coli strain. The enzyme catalyzes the incorporation of nucleotides into duplex DNA in the 5' to 3´  direction in the presence of Mg2+ at 70-80°C. rTaq Plus DNA Polymerase has no detectable exonuclease activity (5' to 3´ or 3' to 5´) and generates a 3´-dA overhang, suitable for TA cloning of PCR products.

rTaq Plus DNA Polymerase is free from any nucleic acid which can be contaminated from expression host during purification process. That provides robust conditions for amplification of gram-negative bacterial genes, and profiling of these.

rTaq Plus DNA Polymerase is provided with 10x optimized reaction buffer.

Unique Features

Suitable for a long PCR (up to 10 kbp)

Highly processive (10 kbp in 60 sec extension) : provide extremely short running time

High yield compared to  common Taq

Applications

Long PCR

gDNA PCR

RT-PCR

Colony PCR

Multiplex PCR

Storage Buffer

5 unit/ml in 50 mM Tris-HCl, pH 8.0, 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.1% NP-40, 0.1% Tween 20, 50% Glycerol.

Unit Definition

One unit of enzyme catalyzes the incorporation of 10 nmol of dNTP into a polynucleotide fraction in 30 min at 72°C.

10x Reaction Buffer

500 mM Tris-HCl, pH 8.0, 100 mM KCl, 60 mM (NH4)2SO4, 15 mM MgCl2, 1% Triton X-100, 0.1 mg/ml BSA.

QC tests

Activity, SDS-PAGE purity, performance tests, genomic DNA contamination test, confirmation test for the absence of endo and exonucleases.

Storage Condition

Store at -20℃.

 

    

 

 

Contact Us

PARTNERSHIP

LOCATION

e-CATALOG

All PRODUCTS

CopyrightⓒELPIS-BIOTECH. Inc. All Rights Reserved.  

Contact elpis@elpisbio.com for more Information.