Pfu Plus DNA Polymerase

 

Products	Qty.	Cat. No.	Conc.	Price(W)	Protocol	MSDS
Pfu Plus DNA Polymerase	250 units	EBT-1401	5 unit/ml	120,000
	500 units	EBT-1402	5 unit/ml	200,000
Description Pfu Plus DNA Polymerase is suitable for a reliable amplification of long (up to 12 kbp) and complex targets with a robust yield and a high accuracy. Although the error rate is slightly lower than that of Pfu DNA Polymerase, the amplification efficiency is about 3-5 times more. The amplified products by Pfu Plus DNA Polymerase can be used for a gene cloning with decreased error rate, and for a site-specific mutagenesis. Pfu Plus DNA Polymerase, like any other polymerases showing proof-reading activity, generates a PCR product with blunt end. Pfu Plus DNA Polymerase exhibits 3' to 5'  exonuclease (proof-reading) activity, but has no detectable 5' to 3´ exonuclease activity. Pfu Plus DNA Polymerase is provided with 10x optimized reaction buffer. Applications • DNA amplification for a gene cloning require high fidelity • Point mutagenesis by PCR using whole vector as a template Storage Buffer 5 unit/ml in 50 mM Tris-HCl, pH 8.2, 0.1 mM EDTA, 1 mM DTT, 50% Glycerol. Unit Definition One unit of enzyme catalyzes the incorporation of 10 nmole of dNTP into a polynucleotide fraction in 30 min at 72°C. 10x Reaction Buffer 200 mM Tris-HCl, pH 9.0, 100 mM KCl, 100 mM (NH4)2SO4, 20 mM MgSO4, 1% Triton X-100, 1 mg/ml BSA. QC tests Activity, SDS-PAGE purity, performance tests, genomic DNA contamination test, confirmation test for the absence of endo and exonucleases. Storage Condition Store at -20℃.