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 Pfu Plus DNA Polymerase

 

Products

Qty.

Cat. No.

Conc.

Price(W)

Protocol

MSDS

 Pfu Plus DNA Polymerase

250 units

EBT-1401

5 unit/ml

120,000

500 units

EBT-1402

5 unit/ml

200,000

 

Description

Pfu Plus DNA Polymerase is suitable for a reliable amplification of long (up to 12 kbp) and complex targets with a robust yield and a high accuracy. Although the error rate is slightly lower than that of Pfu DNA Polymerase, the amplification efficiency is about 3-5 times more. The amplified products by Pfu Plus DNA Polymerase can be used for a gene cloning with decreased error rate, and for a site-specific mutagenesis. Pfu Plus DNA Polymerase, like any other polymerases showing proof-reading activity, generates a PCR product with blunt end.

Pfu Plus DNA Polymerase exhibits 3' to 5'  exonuclease (proof-reading) activity, but has no detectable 5' to 3´ exonuclease activity.

Pfu Plus DNA Polymerase is provided with 10x optimized reaction buffer.

Applications

DNA amplification for a gene cloning require high fidelity

Point mutagenesis by PCR using whole vector as a template

Storage Buffer

5 unit/ml in 50 mM Tris-HCl, pH 8.2, 0.1 mM EDTA, 1 mM DTT, 50% Glycerol.

Unit Definition

One unit of enzyme catalyzes the incorporation of 10 nmole of dNTP into a polynucleotide fraction in 30 min at 72°C.

10x Reaction Buffer

200 mM Tris-HCl, pH 9.0, 100 mM KCl, 100 mM (NH4)2SO4, 20 mM MgSO4, 1% Triton X-100, 1 mg/ml BSA.

QC tests

Activity, SDS-PAGE purity, performance tests, genomic DNA contamination test, confirmation test for the absence of endo and exonucleases.

Storage Condition

Store at -20℃.

 

   

 

 

 

 

 

 

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