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 rTaq Plus HOT DNA  Polymerase

 

Products

Qty.

Cat. No.

Conc.

Price(W)

Protocol

MSDS

 rTaq Plus HOT DNA  Polymerase

250 units

EBT-1611

5 unit/ml

80,000

500 units

EBT-1612

5 unit/ml

140,000

 

Description

rTaq Plus HOT DNA Polymerase is a highly specific DNA polymerase adapting hot start PCR procedure. The whole activity is completely inhibited at low temperature and recovered by exposure to high temperature during initial denaturation step.  It is suitable for an amplification with complicated samples and extremely low concentrated targets. Inhibition of primer dimer formation during PCR process enables easy application to Multiplex PCR and Real-time PCR using SYBR green.

Due to a high elongation rate (about 100 bp/sec) and high processivity (about 250 bases), long sized products can be generated within relatively shorter running time compared to any other commercially available enzymes (10 kbp within 60 sec extension).

Recombinant rTaq Plus HOT DNA Polymerase is purified from a modified recombinant E.coli strain. The enzyme catalyzes the incorporation of nucleotides into duplex DNA in the 5' to 3´  direction in the presence of Mg2+ at 70-80°C. rTaq Plus HOT DNA Polymerase has no detectable exonuclease activity (both 5' to 3´ and 3' to 5´) and generates a 3´-dA overhang, suitable for TA cloning of PCR products.

rTaq Plus HOT DNA Polymerase is free from any nucleic acid which can be contaminated from expression host during purification process. That provides robust conditions for amplification of gram-negative bacterial genes, and profiling of these.

rTaq Plus HOT DNA Polymerase is provided with 10x optimized reaction buffer.

Unique Features

Suitable for a long PCR (up to 10 kbp)

Reduced non-specific amplification and inhibition of primer dimer formation during the first temperature increasing step

Highly processive (10 kbp in 60 sec extension) : provide extremely short running time

High yield compared to common Taq products

Applications

Long PCR

gDNA PCR

RT-PCR

Colony PCR

Multiplex PCR

Real-time PCR using SYBR green

Storage Buffer

5 unit/ml in 50 mM Tris-HCl, pH 8.0, 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.1% NP-40, 0.1% Tween 20, 50% Glycerol.

Unit Definition

One unit of enzyme catalyzes the incorporation of 10 nmol of dNTP into a polynucleotide fraction in 30 min at 72°C.

10x Reaction Buffer

Tris-HCl buffer with NH4, contain 25 mM MgCl2.

QC tests

Activity, SDS-PAGE purity, performance tests, genomic DNA contamination test, confirmation test for the absence of  endo and exonucleases.

Storage Condition

Store at -20℃.

 

  

 

 

 

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