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from GENE to PROTEIN |
from GENE to PROTEIN |
from GENE to PROTEIN |
from GENE to PROTEIN |
from GENE to PROTEIN |
from GENE to PROTEIN |
PROTEIN BIOCHEMISTRY |
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MOLECULAR BIOLOGY |
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Real-Time PCR 2x Master Mix (SYBR green)
Products |
Qty. |
Cat. No. |
Conc. |
Price(W) |
Protocol |
MSDS |
HiPi Real-Time PCR 2x Master Mix (SYBR green) |
1 ml |
EBT-1801 |
100 rxn/20ml |
60,000 |
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5x1 ml |
500 rxn/20ml |
240,000 |
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HiPi Real-Time PCR 2x Master Mix (SYBR green) is a mixture of thermostable DNA polymerase, dNTPs, SYBR green I dye, reaction buffer, and stabilizer. Users can perform real-time PCR by simply adding template cDNA or gDNA and specific primer pair to the master mix. HiPi Real-Time PCR 2x Master Mix (SYBR green) has been optimized for highly sensitive and reproducible real-time PCR experiments. The composition has been optimized to reduce non-specific amplification and to minimize primer-dimer formation. HiPi Real-Time PCR 2x Master Mix (SYBR green) is provided as a 2x concentrated format. Experiment can be simply set by mixing half volume of 2x master mix with primer pair/template DNA solution. 1 ml 2x master mix is sufficient for 100 reactions in a 20 ml standard reaction. ROX reference dye containing product (EBT-1802) is suitable for a real-time PCR instruments requiring ROX passive reference dye (like company ABI real-time PCR machines). The use of ROX reference dye is optional. If your real-time machine has the capability of using ROX and you wish to use this option, then you can select this option in the software.
Real-Time quantitative RT-PCR and genomic DNA PCR.
Performance tests.
Store at -20℃ for one year. After thawing mix, do not freeze again and store at 4℃ in a light protective condition. When stored under optimum conditions, the reagents are stable for a minimum of 6 months from date of purchase.
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Contact elpis@elpisbio.com for more Information.