Real-Time PCR 2x Master Mix (SYBR green)

 

Products	Qty.	Cat. No.	Conc.	Price(W)	Protocol	MSDS
HiPi Real-Time PCR 2x Master Mix (SYBR green)	1 ml	EBT-1801	100 rxn/20ml	60,000
	5x1 ml		500 rxn/20ml	240,000
Description HiPi Real-Time PCR 2x Master Mix (SYBR green) is a mixture of thermostable DNA polymerase, dNTPs, SYBR green I dye, reaction buffer, and stabilizer. Users can perform real-time PCR by simply adding template cDNA or gDNA and specific primer pair to the master mix. HiPi Real-Time PCR 2x Master Mix (SYBR green) has been optimized for highly sensitive and reproducible real-time PCR experiments. The composition has been optimized to reduce non-specific amplification and to minimize primer-dimer formation. HiPi Real-Time PCR 2x Master Mix (SYBR green)  is provided as a 2x concentrated  format. Experiment can be simply set by mixing half volume of 2x master mix with primer pair/template DNA solution. 1 ml 2x master mix is sufficient for 100 reactions in a 20 ml standard reaction. ROX reference dye containing product (EBT-1802)  is suitable for a real-time PCR instruments requiring ROX passive reference dye (like company ABI real-time PCR machines).  The use of ROX reference dye is optional. If your real-time machine has the capability of using ROX and you wish to use this option, then you can select this option in the software. Applications Real-Time quantitative RT-PCR and genomic DNA PCR. QC tests Performance tests. Storage Condition Store at -20℃ for one year. After thawing mix, do not freeze again and store at 4℃ in a light protective condition.  When stored under optimum conditions, the reagents are stable for a minimum of 6 months from date of purchase.