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 Real-Time PCR 2x Master Mix (SYBR green, contain ROX dye)

 

Products

Qty.

Cat. No.

Conc.

Price(W)

Protocol

MSDS

 HiPi Real-Time PCR 2x Master Mix (SYBR green, contain ROX dye)

1 ml

EBT-1802

100 rxn/20ml

60,000

5x1 ml

500 rxn/20ml

240,000

 

Description

HiPi Real-Time PCR 2x Master Mix (SYBR green) is a mixture of thermostable DNA polymerase, dNTPs, SYBR green I dye, reaction buffer, and stabilizer. Users can perform real-time PCR by simply adding template cDNA or gDNA and specific primer pair to the master mix. HiPi Real-Time PCR 2x Master Mix (SYBR green) has been optimized for highly sensitive and reproducible real-time PCR experiments. The composition has been optimized to reduce non-specific amplification and to minimize primer-dimer formation.

HiPi Real-Time PCR 2x Master Mix (SYBR green)  is provided as a 2x concentrated  format. Experiment can be simply set by mixing half volume of 2x master mix with primer pair/template DNA solution. 1 ml 2x master mix is sufficient for 100 reactions in a 20 ml standard reaction.

ROX reference dye containing product (EBT-1802)  is suitable for a real-time PCR instruments requiring ROX passive reference dye (like company ABI real-time PCR machines).  The use of ROX reference dye is optional. If your real-time machine has the capability of using ROX and you wish to use this option, then you can select this option in the software.

Applications

Real-Time quantitative RT-PCR and genomic DNA PCR.

QC tests

Performance tests.

Storage Condition

Store at -20℃ for one year. After thawing mix, do not freeze again and store at 4℃ in a light protective condition.  When stored under optimum conditions, the reagents are stable for a minimum of 6 months from date of purchase.

 

   

 

 

 

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