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from GENE to PROTEIN

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from GENE to PROTEIN

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 Rapid Ligaiton 2x PreMix

 

Products

Qty.

Cat. No.

Conc.

Price(W)

Protocol

Brochure

 Rapid Ligation 2x Premix

96 tubes

EBT-2002

5 ml/tube

160,000

 

 

Description

T4 DNA Ligase catalyzes the joining of two strands of DNA between the 5´-phosphate and the 3´-hydroxyl groups of adjacent nucleotides in either a cohesive-ended or blunt-ended configuration. Rapid Ligation 2x PreMix contains T4 DNA Ligase, optimized ligation buffer, and stabilizer in 200 μl thin-wall PCR tubes. The ligation of cohesive-ended or blunt-ended DNA mixed with vector is following by incubation for 5-30 min at 37°C. The efficiency of ligation is about 80%.

2x Master Mix or PreMix

2x Rapid Ligation PreMix contains T4 DNA Ligase, 2x ligation buffer, and stabilizer in 5 μl  premix (final 10 μl total reaction). Use with 5´, 3´ or blunt-ended DNA inserts.

Recommended Storage Condition

Store at -20°C.

Do not freeze and thaw frequently.  Doing this may decrease ligation efficiency.

Applications

Joining double-stranded DNA molecules with cohesive- or blunt-ends.

 

 

 

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