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from GENE to PROTEIN

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from GENE to PROTEIN

from GENE to PROTEIN

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MOLECULAR BIOLOGY

 

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 Klenow Fragment, exo-

 

Products

Qty.

Cat. No.

Conc.

Price(W)

Protocol

MSDS

 Kelnow Fragment, exo-

200 unit

EBT-3000

5 unit/ml

80,000

 

Description

DNA Polymerase I Large (Klenow -exo) Fragment is a DNA-dependent DNA polymerase that lacks both the 5' to 3´ and 3' to 5’ exonuclease activity of intact E. coli DNA Polymerase I.  DNA Polymerase I Large (Klenow -exo) Fragment is used for random priming and chain displacement amplification. But it is not recommended in blunt formation for blunt-ended ligation.

DNA Polymerase I Large (Klenow -exo) Fragment is purified from a recombinant E.coli strain.

Concentration & Storage Condition

5 unit/ml.  Store at -20℃.

Storage Buffer

50 mM Tris-HCl, pH 7.5, 1 mM DTT, 0.1 mM EDTA, 50% (v/v) glycerol.

10x Reaction Buffer

500 mM Tris-HCl, pH 7.2, 100 mM MgSO4, 1 mM DTT.

Unit Definition

One unit is defined as the amount of enzyme that incorporates 10 nmole of total dNTP into TCA-insoluble material in 30 min at 37°C. The reaction conditions are: 67 mM potassium phosphate, pH 7.5, 6.7 mM MgCl2, 1 mM DTT, 50 mg/ml activated calf thymus DNA and 33 mM each of dATP, dCTP, dGTP and dTTP (a mix of unlabeled and [3H]dTTP).

QC Tests

Activity, exo and endonuclease activity test, SDS-PAGE purity, performance tests.  

 

 

 

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