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 Phi6 RNA Polymerase

 

Products

Qty.

Cat. No.

Conc.

Price(W)

Protocol

MSDS

 Phi6 RNA Polymerase

100 unit

EBT-3003

1 unit/ml

150,000

 

Description

The Phi6 RNA Polymerase is an RNA-dependent RNA polymerase (RdRp) of bacteriophage Phi6. It is a highly processive enzyme which synthesizes a full length complementary RNA strand using either single or double stranded RNA as templates. The RNA synthesis initiates from the 3´ end of template RNA without primer. The recombinant enzyme is purified from E. coli strain that carries a P2 gene of bacteriophage Phi6.

Applications

- Synthesis of dsRNA in vitro

- Synthesis of dsRNA for RNAi

- Primer independent RNA synthesis

Reagents Supplied & Storage Condition

- Phi6 RNA Polymerase : 1 unit/ml,  Store at -20°C.

- 10x Phi6 RNA Pol Reaction Buffer :  Store at 4°C.

- 50 mM MnCl2 :  Store at 4°C.

- ATP, UTP, CTP, GTP are not included in the product.

Reaction Condition

1X Phi6  RNA Pol Reaction Buffer  with 1.5 mM MnCl2, 0.1-0.2 mM each ATP, UTP, CTP and 0.3-0.6 mM GTP. Incubate at 32°C.

10x Reaction Buffer

500 mM Tris-HCl, pH8.9, 500 mM Ammonium Acetate.

Storage Buffer

50 mM Tris-HCl, pH8.0. 100 mM NaCl, 0.1 mM EDTA, 0.1% Triton X-100, 50% Glycerol.

Unit  Definition

One unit is defined as the amount of enzyme that will incorporate 1 nmol of UTP into an acid-insoluble material in 20 minutes at 32°C.

QC Tests

Activity, exo and endonuclease activity test, SDS-PAGE purity, performance tests.

 

 

 

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