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 Phi29 DNA Polymerase

 

Products

Qty.

Cat. No.

Conc.

Price(W)

Protocol

MSDS

 Phi29 DNA Polymerase

500 unit

EBT-3004

10 unit/ml

150,000

 

Description

Phi29 DNA Polymerase is one of the enzymes showing strong strand displacement DNA synthetic activity. The polymerase has an inherent 3' to 5´ proof-reading exonuclease activity. The polymerase gene from the Bacillus subtilis phage phi29 (Φ29) was overexpressed and purified from E.coli strain.

  - Extreme strand displacement : best fit for RCA (rolling circle amplification) and WGA by MDA (whole genome amplification by multiple displacement amplification

  - High fidelity : 3' to 5´ proof-reading exonuclease activity

  - Extreme processivity : up to 70 kbp

Concentration & Storage Condition

10 unit/ml.  Store at -20℃.

Storage Buffer

10 mM Tris-HCl, pH 7.5, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.1% Triton X-100, 50% glycerol.

10x Reaction Buffer

500 mM Tris-HCl, pH 7.5, 100 mM (NH4)2SO4, 100 mM MgCl2,  40 mM DTT.

Unit Definition

One unit is defined as the amount of enzyme required to incorporate 0.5 pmol of dNTP into acid insoluble material in 10 minutes at 30°C

QC Tests

Activity, exo and endonuclease activity test, DNA contamination test, SDS-PAGE purity, performance tests. 

 

  

 

 

 

 

 

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