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 Bsu DNA Polymerase

 

Products

Qty.

Cat. No.

Conc.

Price(W)

Protocol

MSDS

 Bsu DNA Polymerase

250 unit

EBT-3005

5 unit/ml

120,000

 

Description

Bsu DNA Polymerase I is a DNA-dependent DNA polymerase  lacks the 5´à3´ and  à5´ exonuclease activities. This polymerase has a strong strand displacement activity.  Bsu DNA Polymerase I gene is obtained from Bacillus subtilis  and the polymerase is purified from a recombinant E.coli strain.

Concentration & Storage Condition

5 unit/ml.  Store at -20℃.

Storage Buffer

50 mM Tris-HCl, pH 7.5, 1 mM DTT, 0.1 mM EDTA, 50% (v/v) glycerol.

10x Reaction Buffer

100 mM Tris-HCl, pH 7.5, 500 mM NaCl, 100 mM MgCl2, 10 mM DTT.

Unit Definition

One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol of dNTPs into acid insoluble material in 30 min at 37°C.

QC Tests

Activity, exo and endonuclease activity test, DNA contamination test, SDS-PAGE purity, performance tests.   

 

 

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