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 Lambda Exonuclease

 

Products

Qty.

Cat. No.

Conc.

Price(W)

Protocol

Brochure

 Lambda Exonuclease

1,000 unit

EBT-3020

5 unit/ml

60,000

 

Description

Lambda Exonuclease catalyzes the removal of 5´-mononucleotides from duplex DNA  (5´ to 3´). The preferred substrate is 5´-phosphorylated double-stranded DNA, although it will also degrade single-stranded and non-phosphorylated substrates at a greatly reduced rate. Lambda Exonuclease is unable to initiate DNA digestion at nicks or gaps.

Concentration & Storage Condition

5 unit/ml.  Store at -20℃.

Storage Buffer  

25 mM Tris-HCl, pH 8.0, 50 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 50 μg/ml BSA and 50% glycerol.

10x Reaction Buffer

670 mM Glycine-KOH, pH 9.4,  25 mM MgCl2 and 500 mg/ml BSA. 

Unit Definition  

One unit is defined as the amount of enzyme required to produce 10 nmole of acid-soluble dNTP from double-stranded substrate in a total reaction volume of 50 ml in 30 min at 37℃ in 1x Lambda Exonuclease reaction buffer with 1 mg sonicated duplex [3H]-DNA.

QC Tests  

Activity, exo and endonuclease activity test, SDS-PAGE purity, performance tests.   

 

 

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