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from GENE to PROTEIN

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from GENE to PROTEIN

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PROTEIN BIOCHEMISTRY

 

MOLECULAR BIOLOGY

 

Prestained Protein Markers

 

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 RNase I

 

Products

Qty.

Cat. No.

Conc.

Price(W)

Protocol

MSDS

 RNase I

10,000 unit

EBT-3021

100 unit/ml

80,000

 

Description

RNase I ribonuclease catalyzes the degradation of RNA to cyclic nucleotide monophosphate intermediates. RNase I ribonuclease is one of the few known RNases that can cleave a phosophodiester bond between any two ribonucleotides. RNase I ribonuclease may be used 1) to remove RNA from DNA preparations, 2) for RNase protection assays and 3) for mapping or quantitation of RNA by selective cleavage of single-stranded regions. RNase I is purified from a recombinant E.coli strain.

Concentration & Storage Condition

100 unit/ml.  Store at -20℃.

Storage Buffer  

10 mM Tris-HCl, pH 8.0, 200 mM NaCl, 50% glycerol.

10x Reaction Buffer

100 mM Tris-HCl, pH 7.5, 50 mM EDTA, 2 M sodium acetate.

Unit Definition  

One unit is defined as the amount of enzyme required to completely degrade RNA at the rate of 100 ng/sec at 37°C in 10 mM Tris-HCl, pH 7.5, 5 mM EDTA, 200 mM sodium acetate, 0.2 mg RNA, 0.05% NP-40 and 2 mg BSA.

QC Tests  

Activity, exo and endonuclease activity test, SDS-PAGE purity, performance tests.   

 

 

 

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