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from GENE to PROTEIN |
from GENE to PROTEIN |
from GENE to PROTEIN |
from GENE to PROTEIN |
from GENE to PROTEIN |
from GENE to PROTEIN |
PROTEIN BIOCHEMISTRY |
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MOLECULAR BIOLOGY |
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T7 Endonuclease
Products |
Qty. |
Cat. No. |
Conc. |
Price(W) |
Protocol |
MSDS |
T7 Endonuclease I |
500 unit |
EBT-3027 |
10 unit/ml |
120,000 |
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T7 Endonuclease I, product of T7 gene 3, also known as junction-resolving enzyme, selectively binds and cleaves cruciform DNA structures, four-way (Holliday structures) DNA junctions, heteroduplex DNA and nicked double-stranded DNA. The cleavage site is at the first, second or third phosphodiester bond that is 5´ to the mismatch. T7 Endonuclease I is expressed and purified from E.coli.
- Resolve four-way junction or branched DNA. - Detect or cleave heteroduplex and nicked DNA. - Randomly cleave linear DNA for shot-gun cloning.
- T7 Endonuclease I : 10 unit/㎕, Store at -20°C. - 10x T7 Endonuclease I Reaction Buffer : Store at 4°C.
T7 Endonuclease I in 1X T7 Endonuclease I Reaction Buffer. Incubate at 37°C.
100 mM Tris-HCl (pH 7.9), 500 mM NaCl, 100 mM MgCl2, 10 mM DTT
20 mM Tris-HCl (pH 7.5), 200 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, 0.1% Triton® X-100
One unit is defined as the amount of enzyme required to convert > 90% of 1 μg of supercoiled cruciform pUC(AT) to > 90% linear form in a total reaction volume of 50 μl in 1 hour at 37°C.
Activity, exo and endonuclease activity test, SDS-PAGE purity, performance tests.
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