T7 Endonuclease

 

Products	Qty.	Cat. No.	Conc.	Price(W)	Protocol	MSDS
T7 Endonuclease I	500 unit	EBT-3027	10 unit/ml	120,000
Description T7 Endonuclease I, product of T7 gene 3, also known as junction-resolving enzyme, selectively binds and cleaves cruciform DNA structures, four-way (Holliday structures) DNA junctions, heteroduplex DNA and nicked double-stranded DNA. The cleavage site is at the first, second or third phosphodiester bond that is 5´ to the mismatch.  T7 Endonuclease I is expressed and purified from E.coli. Applications - Resolve four-way junction or branched DNA. - Detect or cleave heteroduplex and nicked DNA. - Randomly cleave linear DNA for shot-gun cloning. Reagents Supplied & Storage Condition - T7 Endonuclease I : 10 unit/㎕,  Store at -20°C. - 10x T7 Endonuclease I Reaction Buffer : Store at 4°C. Reaction Condition T7 Endonuclease I in 1X T7 Endonuclease I Reaction Buffer. Incubate at 37°C. 10x Reaction Buffer 100 mM Tris-HCl (pH 7.9), 500 mM NaCl, 100 mM MgCl2, 10 mM DTT Storage Buffer 20 mM Tris-HCl (pH 7.5), 200 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, 0.1% Triton® X-100 Unit  Definition One unit is defined as the amount of enzyme required to convert > 90% of 1 μg of supercoiled cruciform pUC(AT) to > 90% linear form in a total reaction volume of 50 μl in 1 hour at 37°C. QC Tests Activity, exo and endonuclease activity test, SDS-PAGE purity, performance tests.