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 T4 Polynucleotide Kinase

 

Products

Qty.

Cat. No.

Conc.

Price(W)

Protocol

Brochure

 T4 Polynucleotide Kinase

500 unit

EBT-3031

10 unit/ml

80,000

 

 

Description

 T4 Polynucleotide Kinase catalyzes the transfer of the g-phosphate from ATP to the 5´-terminus of polynucleotides or to mononucleotides bearing a 5´-hydroxyl group. The enzyme can be used to phosphorylate RNA, DNA and synthetic oligonucleotides. T4 Polynucleotide Kinase is purified from a recombinant E. coli strain.

Concentration & Storage Condition

10 unit/ml.  Store at -20℃.

Storage Buffer  

20 mM Tris-HCl, pH 7.5, 25 mM KCl, 2 mM DTT, 0.1 mM EDTA, 0.1 mM ATP and 50% glycerol.

10x Reaction Buffer

700 mM Tris-HCl, pH 7.6, 100 mM MgCl2, 50 mM DTT.

Unit Definition  

One unit is defined as the amount of enzyme required to catalyze the transfer of 1 nmole of phosphate to the 5´-OH end of a polynucleotide from [g-32P]ATP in 30 min at 37°C. The reaction conditions are: 40 mM Tris-HCl, pH 7.5, 10 mM MgCl2, 5 mM DTT, 0.1 mM [g-32P] ATP and 0.5 mM 5´-OH polynucleotide end concentration.

QC Tests  

Activity, exo and endonuclease activity test, SDS-PAGE purity, performance tests. 

 

 

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