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 Bacterial Alkaline Phosphatase

 

Products

Qty.

Cat. No.

Conc.

Price(W)

Protocol

MSDS

 Bacterial Alkaline Phosphatase

100 unit

EBT-3032

0.5 unit/ml

60,000

 

Description

Bacterial Alkaline Phosphatase catalyzes the hydrolysis of 5´-phosphate groups from DNA, RNA, and ribo- and deoxyribonucleoside triphosphates. This enzyme is used to prevent recircularization and religation of linearized cloning vector DNA by removing phosphate groups from both 5´-termini and may also be used for the dephosphorylation of 5´ phosphorylated ends of DNA or RNA for subsequent labeling with [g-32P]ATP and T4 Polynucleotide Kinase. Bacterial Alkaline Phosphatase is purified from a recombinant E.coli strain.

Concentration & Storage Condition

0.5 unit/ml.  Store at -20℃.

Storage Buffer  

50 mM Tris-HCl, pH 8.0, 100 mM KCl, 1 mM MgSO4, 50% glycerol.

10x Reaction Buffer

500 mM Tris-HCl, pH 9.0, 10 mM MgCl2.

Unit Definition  

One unit is defined as the amount of enzyme required to catalyze the hydrolysis of 1 mmol of 4-nitrophenyl phosphate per min at 37°C in 1 M diethanolamine, 10.9 mM 4-nitrophenyl phosphate, 0.5 mM MgCl2, pH 9.8.

QC Tests  

Activity, exo and endonuclease activity test, SDS-PAGE purity, performance tests.

 

 

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