www.elpisbio.com

 

 

intranet

 

 

 

 

 

 

 

 

HOME

PRODUCTS

SUPPORT

Q & A

USEFUL SITES

About Us

from GENE to PROTEIN

from GENE to PROTEIN

from GENE to PROTEIN

from GENE to PROTEIN

from GENE to PROTEIN

from GENE to PROTEIN

 

 

 

PROTEIN BIOCHEMISTRY

 

MOLECULAR BIOLOGY

 

Prestained Protein Markers

 

DNA Size Ladder Markers

 

Unstained Protein Markers

 

PCR Enzymes

 

Western Markers

 

PCR Buffer & Additives

 

Protein Gel Electrophoresis Kits

 

PCR Master Mix & Premixes

 

Gel Electrophoresis Reagents

 

cDNA Synthesis Enzymes

 

Premade Buffers

 

RT Master Mixes

 

Protein Gel Stains

 

Gene Cloning Kits

 

Protein Purification Resin & Kit

 

Site-specific Mutagenesis Kit

 

Western Detection Reagents

 

Polymerases

 

Site-specific Endoproteases

 

Endo-, Exo-Nucleases

 

Endo-, Exo-glycosidases

 

Ligase/Kinase/Phosphatase

 

Protein Enzymes

 

Recombinases

 

 

 

Restriction Enzymes

 

Human Proteins

 

Nucleic Acid Purification Kits

 

 

 

Reagents & Buffers

 

 

 

Modification Enzymes

 

 

 

 

 Exo53 (5' to 3' Exonuclease)

 

Products

Qty.

Cat. No.

Conc.

Price(W)

Protocol

MSDS

 Exo53 (5' to 3' Exonuclease)

500 unit

EBT-3047

10 unit/ml

150,000

 

Description

5'-3' Exonuclease (Exo53) catalyzes the removal of 5´ mononucleotides from duplex DNA  (5' to 3´ exonuclease avtivity). Unlike lambda exonuclease, Exo53 degrades both 5´-phosphorylated and 5'-dephosphorylated double stranded DNA. Exo53 is unable to initiate DNA digestion at nicks or gaps. Exo53 is purified from E.coli strain containing gene of exonuclease domain of E.coli DNA pol I.

Concentration & Storage Condition

10 unit/ml,  Store at -20℃.

10x Reaction Buffer

100 mM Tris-HCl, pH7.9, 500 mM NaCl, 100 mM MgCl2, 10 mM DTT.

Storage Buffer

25 mM Tris-HCl, pH 8.0, 50 mM NaCl, 1 mM DTT, 0.1 mM EDTA, and 50% glycerol.

Unit Definition

One unit is defined as the amount of enzyme required to produce 1 nmole of acid-soluble nucleotides from double-stranded DNA within 1 hr at 37°C.

QC TestsActivity, exo and endonuclease activity test, SDS-PAGE purity, performance tests.

 

 

 

Contact Us

PARTNERSHIP

LOCATION

e-CATALOG

All PRODUCTS

CopyrightⓒELPIS-BIOTECH. Inc. All Rights Reserved.  

Contact elpis@elpisbio.com for more Information.