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from GENE to PROTEIN |
from GENE to PROTEIN |
from GENE to PROTEIN |
from GENE to PROTEIN |
from GENE to PROTEIN |
from GENE to PROTEIN |
PROTEIN BIOCHEMISTRY |
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MOLECULAR BIOLOGY |
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Exo53 (5' to 3' Exonuclease)
Products |
Qty. |
Cat. No. |
Conc. |
Price(W) |
Protocol |
MSDS |
Exo53 (5' to 3' Exonuclease) |
500 unit |
EBT-3047 |
10 unit/ml |
150,000 |
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5'-3' Exonuclease (Exo53) catalyzes the removal of 5´ mononucleotides from duplex DNA (5' to 3´ exonuclease avtivity). Unlike lambda exonuclease, Exo53 degrades both 5´-phosphorylated and 5'-dephosphorylated double stranded DNA. Exo53 is unable to initiate DNA digestion at nicks or gaps. Exo53 is purified from E.coli strain containing gene of exonuclease domain of E.coli DNA pol I.
10 unit/ml, Store at -20℃.
100 mM Tris-HCl, pH7.9, 500 mM NaCl, 100 mM MgCl2, 10 mM DTT.
25 mM Tris-HCl, pH 8.0, 50 mM NaCl, 1 mM DTT, 0.1 mM EDTA, and 50% glycerol.
One unit is defined as the amount of enzyme required to produce 1 nmole of acid-soluble nucleotides from double-stranded DNA within 1 hr at 37°C.
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