Bst DNA Polymerase exo- is a recombinant DNA polymerase
from Bacillus stearothermophilus. Bst DNA Polymerase is purified from an E.coli
strain carrying a plasmid with the cloned gene encoding large fragment of DNA
polymerase. It contains the 5´-> 3´ polymerase activity, but lacks 5´->
3´ exonuclease activity. For this reason, Bst DNA Polymerase can be effectively
used in chain displacement reaction at high temperature, 65°C.
- Isothermal amplification
- Applications requiring
strand-displacement DNA synthesis
- DNA sequencing through high
- Rapid sequencing from
nanogram amounts of DNA template
120 unit/㎕ in 10 mM Tris-HCl, pH8.0, 50 mM KCl, 0.1 mM EDTA, 1 mM
DTT, 0.1% Triton X-100, and 50% Glycerol
10x Reaction Buffer
200 mM Tris-HCl, pH8.8, 100 mM
(NH4)2SO4, 700 mM KCl, 30 mM MgSO4, 1% Tween20.
One unit of enzyme
incorporates 10 nmole of dNTP into acid insoluble material in 30 min at 65°C.
Activity, SDS-PAGE purity,
performance tests, genomic DNA contamination test, confirmation test for the
absence of endo and exonucleases.
Store at -20℃.
LAMP : Lambda DNA
LAMP : Salt, Mg Optimization
LAMP : Comparison with Bst2.0