Bst DNA Polymerase exo- is a recombinant DNA polymerase from Bacillus stearothermophilus. Bst DNA Polymerase is purified from an E.coli strain carrying a plasmid with the cloned gene encoding large fragment of DNA polymerase. It contains the 5´-> 3´ polymerase activity, but lacks 5´-> 3´ exonuclease activity. For this reason, Bst DNA Polymerase can be effectively used in chain displacement reaction at high temperature, 65°C.

 

Applications

 - Isothermal amplification (LAMP)

 - Applications requiring strand-displacement DNA synthesis

 - DNA sequencing through high GC regions

 - Rapid sequencing from nanogram amounts of DNA template

 

Storage Buffer

  120 unit/㎕ in 10 mM Tris-HCl, pH8.0, 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.1% Triton X-100, and 50% Glycerol

 

10x Reaction Buffer

  200 mM Tris-HCl, pH8.8, 100 mM (NH₄)₂SO₄, 700 mM KCl, 30 mM MgSO₄, 1% Tween20.

 

Unit Definition

  One unit of enzyme incorporates 10 nmole of dNTP into acid insoluble material in 30 min at 65°C.

 

QC Tests

  Activity, SDS-PAGE purity, performance tests, genomic DNA contamination test, confirmation test for the absence of endo and exonucleases.

 

 

Storage Condition

  Store at -20℃.

 

 

 

LAMP : Lambda DNA

 LAMP : Salt, Mg Optimization        

LAMP : Comparison with Bst2.0