Bst V2 DNA Polymerase is a recombinant DNA polymerase from Bacillus stearothermophilus, which is engineered for improved isothermal amplification performance. Bst V2 DNA Polymerase is purified from an E.coli strain carrying a plasmid with the cloned gene encoding large fragment of the DNA polymerase.

 

  It contains the 5´→ 3´ polymerase activity, but lacks 5´ → 3´ and 3’ → 5’ exonuclease activity. For this reason, Bst V2 DNA Polymerase can be effectively used in strand displacement reaction at high temperature, 60~70°C.

 

Applications 

 - Isothermal amplification (LAMP, RCA, NASBA, etc.)

 - Applications requiring strand-displacement DNA synthesis

 - DNA sequencing through high GC regions

 - Rapid sequencing from nanogram amounts of DNA template

 

Storage Buffer

 - 8 unit/㎕ in 10 mM Tris-HCl, pH8.0, 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.1% Triton X-100, and 50% Glycerol.

10x Buffer

 - 200 mM Tris-HCl, pH8.8, 150 mM (NH₄)₂SO₄, 1 M KCl, 25 mM MgSO₄, 1% Tween20.

 

Unit Definition

 - One unit of enzyme incorporates 10 nmole of dNTP into acid insoluble material in 30 min at 65°C.

 

QC Tests

- Activity, SDS-PAGE purity, performance tests, genomic DNA contamination test, confirmation test for the absence of endo and exonucleases.

 

Storage Condition

 - Store at -20℃. 

 

 

LAMP : Lambda DNA

 

 

RT-LAMP : ACTB