>

PyroMax DNA Polymerase
More Information
CAT #
EBT-1313
QTY
250 unit (5 unit/㎕)
PRICE (W)
120,000
MANUAL
MSDS
이 분류에 등록된 다른 상품이 없습니다. 확대보기
리뷰 위시 0

상품간략정보 및 구매기능

판매가격 판매중지
배송비결제 주문시 결제

상품의 재고가 부족하여 구매할 수 없습니다.

Description

PyroMax DNA Polymerase is purified from an recombinant E.coli strain.

This enzyme catalyzes the incorporation of nucleotides into duplex DNA in the 5‘=> 3' direction in the presence of Mg²⁺ at 70-80°C. PyroMax DNA Polymerase exhibits 3´-> 5´ exonuclease (proof-reading) activity, but has no detectable 5´-> 3´ exonuclease activity.

This enzyme is designed for a reliable amplification of long, complex targets with robust yield and high accuracy. Although the error rate is similar as Pfu, the amplification speed is about 3-5 times more than Pfu. The amplified products by PyroMax DNA Polymerase can be used for a cloning of your genes with decreased error rate. PyroMax DNA Polymerase, like any other polymerases showing proof-reading activity, generates a PCR products with blunt end.

PyroMax DNA Polymerase is free from any nucleic acid which can be contaminated by expression host during purification process.
That provides robust conditions for amplification of gram-negative bacterial genes, and profiling of these.

PyroMax DNA Polymerase is provided with 10x optimized reaction buffer.

Storage Buffer
- 5 unit/㎕ in 50 mM Tris-HCl, pH 8.0, 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.1% NP-40, 0.1% Tween-20, 50% Glycerol.

Unit Definition
- One unit of enzyme catalyzes the incorporation of 10 nmole of dNTP into a polynucleotide fraction in 30 min at 72°C.

10x Reaction Buffer
- 500 mM Tris-HCl, pH 7.9, 100 mM KCl, 50 mM (NH₄)₂SO₄, 15 mM MgCl₂, 1% Triton X-100, 0.1 mg/ml BSA.

QC tests
- Activity, SDS-PAGE purity, performance tests, absence of endo, and exonucleases.

Storage Condition

 


 

- Store at -20℃. 

FAQ
  • 등록된 내용이 없습니다.
Related Products

선택하신 상품이 장바구니에 담겼습니다.

계속구매 장바구니 이동

사이트 정보

회사명 : 회사명 / 대표 : 대표자명
주소 : OO도 OO시 OO구 OO동 123-45
사업자 등록번호 : 123-45-67890
전화 : 02-123-4567 팩스 : 02-123-4568
통신판매업신고번호 : 제 OO구 - 123호
개인정보관리책임자 : 정보책임자명

Copyright © 소유하신 도메인. All rights reserved.