HiPi Super DNA Polymerase is suitable for an amplification of < 40 kbp DNA fragments with a high fidelity and specificity. It is highly effective in long PCR and multiplex PCR.HiPi Super DNA Polymerase catalyzes the incorporation of nucleotides into duplex DNA in the 5´ to 3´ direction in the presence of Mg²⁺ at 70-80°C. HiPi Super DNA Polymerase is a mix of DNA polymerase showing 3´ to 5´ exonuclease (proof-reading) activity and Taq DNA Polymerase. Due to its high elongation rate, long sized products can be generated within relatively shorter running time compared to any other commercially available enzymes (10 kbp within 30 sec extension).This enzyme is designed for a reliable amplification of long (up to 40 kbp for lambda DNA and 20 kbp for human genomic DNA) and complex targets with a robust yield and a high accuracy. HiPi Super DNA Polymerase generates a mixture of PCR products with blunt end and 3´-dA overhangs.
Applications- PCR for a cloning with reduced error- RT-PCR- Genomic DNA PCR- Long PCR- Highly specific amplification of DNA fragments with high GC content- Multiplex PCR Storage Buffer- 5 unit/㎕ in 20 mM Tris-HCl, pH8.0, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5% Tween 20, and 50% Glycerol. Unit Definition- One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmole of dNTP into an acid-insoluble form in 30 min at 72℃. The reaction conditions are : 25 mM TAPS, pH9.3, 50 mM KCl, 2 mM MgCl₂, 1 mM β-mercaptoethanol, 200 μM each dNTPs, 100 μM [α-³²P]dCTP, 12.5 ㎍ activated calf thymus DNA in a total volume of 50 ㎕. 10x Reaction Buffer- 500 mM Tris-HCl. pH9.0, 160 mM (NH₄)₂SO₄, 35 mM MgCl₂, 1% Triton X-100, 1 mg/ml BSA. 10x Q Buffer- PCR additive for increasing specificity of PCR amplification.Q buffer can be added as 0.5x or 1x to PCR mix. QC tests- Activity, SDS-PAGE purity, performance tests, genomic DNA contamination test, confirmation test for the absence of endo and exonucleases. Storage Condition
- Store at -20℃.