PyroMax DNA Polymerase is purified from an recombinant E.coli strain.
This enzyme catalyzes the incorporation of nucleotides into duplex DNA in the 5‘=> 3' direction in the presence of Mg²⁺ at 70-80°C. PyroMax DNA Polymerase exhibits 3´-> 5´ exonuclease (proof-reading) activity, but has no detectable 5´-> 3´ exonuclease activity.
This enzyme is designed for a reliable amplification of long, complex targets with robust yield and high accuracy. Although the error rate is similar as Pfu, the amplification speed is about 3-5 times more than Pfu. The amplified products by PyroMax DNA Polymerase can be used for a cloning of your genes with decreased error rate. PyroMax DNA Polymerase, like any other polymerases showing proof-reading activity, generates a PCR products with blunt end.
PyroMax DNA Polymerase is free from any nucleic acid which can be contaminated by expression host during purification process.
That provides robust conditions for amplification of gram-negative bacterial genes, and profiling of these.
PyroMax DNA Polymerase is provided with 10x optimized reaction buffer.
Storage Buffer- 5 unit/㎕ in 50 mM Tris-HCl, pH 8.0, 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.1% NP-40, 0.1% Tween-20, 50% Glycerol. Unit Definition- One unit of enzyme catalyzes the incorporation of 10 nmole of dNTP into a polynucleotide fraction in 30 min at 72°C. 10x Reaction Buffer- 500 mM Tris-HCl, pH 7.9, 100 mM KCl, 50 mM (NH₄)₂SO₄, 15 mM MgCl₂, 1% Triton X-100, 0.1 mg/ml BSA. QC tests- Activity, SDS-PAGE purity, performance tests, absence of endo, and exonucleases. Storage Condition- Store at -20℃.