HS Plus Polymerase is a combination of rTaq DNA polymerase, Pfu DNA Polymerase, and a monoclonal antibody against Taq DNA polymerase.

 This antibody inhibits the polymerase activity and prevents non-specific amplification caused by primer dimer mispriming before the PCR cycling starts.

 During the initial denaturation step, the antibody is denatured, allowing the DNA polymerases to function effectively.

 

  Derived from Thermus aquaticus,

HS Plus DNA Polymerase is a recombinant thermostable enzyme that possesses both 5' to 3' exonuclease activity and 3' to 5' exonuclease (proofreading) activity.

Thanks to its proofreading function, it ensures high accuracy in DNA synthesis and is designed for reliable amplification of long and complex targets.

With a high elongation rate of approximately 100 bp/sec and high processivity of about 250 bases,

 HS Plus DNA Polymerase can generate long-sized products in a shorter time compared to other commercially available enzymes.

 

 

Storage Buffer

-  5 unit/㎕ in 50 mM Tris-HCl, pH 8.0, 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.1% NP-40, 0.1% Tween 20, 50% Glycerol.