rTaq Plus 5x PCR Master Mix
More Information
1 ml (250 rxn /20 ㎕)
이 분류에 등록된 다른 상품이 없습니다. 확대보기
리뷰 위시 0

상품간략정보 및 구매기능

판매가격 판매중지
배송비결제 주문시 결제

상품의 재고가 부족하여 구매할 수 없습니다.


rTaq Plus 5x PCR Master Mix is a ready-to-use premix containing all the components essential for a PCR and agarose gel electrophoresis (DNA polymerase, dNTP, reaction buffer, glycerol, bromophenol blue, and stabilizer). PCR can be performed simply by adding primer pair and template.

As Master Mix is supplied as a 5x concentration format, users should adjust a final reaction to 1x (if final reaction volume is 20 ㎕, 4 ㎕ of 5x Master Mix should be added). One unit of rTaq Plus DNA Polymerase is contained in 4 ㎕ of 5x Master Mix.

rTaq Plus DNA Polymerase is a thermostable DNA polymerase that designed for a reliable amplification of long and complex targets with a robust yield in an extremely short extension time. Due to its high elongation rate (about 100 bp/sec) and high processivity (about 250 bases), long sized products can be generated within relatively shorter running time compared to any other commercially available enzymes (5 kbp within 60 sec extension).

Recombinant rTaq Plus DNA Polymerase is purified from a modified recombinant E.coli strain. The enzyme catalyzes the incorporation of nucleotides into duplex DNA in the 5' to 3´ direction in the presence of Mg²⁺ at 70-80°C. rTaq Plus DNA Polymerase has no detectable exonuclease activity (5' to 3´ or 3' to 5´) and generates a 3´dA overhang, suitable for a TA cloning of PCR products.

rTaq Plus DNA Polymerase is free from any nucleic acid which can be contaminated from expression host during purification process. That provides robust conditions for amplification of gram-negative bacterial genes, and profiling of these.

QC tests
- Performance tests, genomic DNA contamination test, confirmation test for the absence of endo and exonucleases.

Storage Condition
- Store at -20°C.
- rTaq Plus 5x PCR Master Mix is stable for at least 2 years at recommended storage condition.

Standard Protocol
1.Prepare 20 ㎕ PCR solution as follows:
PCR grade distilled water : - ㎕
rTaq Plus 5x PCR Master Mix : 4 ㎕
Primer (10 pmol/㎕) : 0.5 ㎕ each
Template DNA : 0.1-10 ng
Adjust final vol. to 20 ㎕ with PCR grade distilled water

2. Set PCR cycling as follows :
Initial denature at 95°C : 3 min
Denature : 95°C, 10 sec
Anneal : Tm-4°C, 10 sec
Extend : 72°C, 10 sec/kbp

* Set 25-35 PCR cycles for effective amplification
* You can also use two step cycle amplification (denaturation at 95°C and annealing/extension at 68°C)

3. You can analyze PCR products by direct loading into agarose gel because PCR Master Mix contains glycerol and bromophenol blue (blue color) essential for a gel loading. 



  • 등록된 내용이 없습니다.
Related Products

선택하신 상품이 장바구니에 담겼습니다.

계속구매 장바구니 이동

사이트 정보

회사명 : 회사명 / 대표 : 대표자명
주소 : OO도 OO시 OO구 OO동 123-45
사업자 등록번호 : 123-45-67890
전화 : 02-123-4567 팩스 : 02-123-4568
통신판매업신고번호 : 제 OO구 - 123호
개인정보관리책임자 : 정보책임자명

Copyright © 소유하신 도메인. All rights reserved.