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EzPCR™ Basic 5x Master Mix
More Information
CAT #
EBT-7151
QTY
1ml (250 rxn / 4㎕)
PRICE (W)
50,000
MANUAL
MSDS
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Description

  EzPCRTM BASIC 5x PCR Master Mix is a ready-to-use premix containing all the components essential for a PCR and agarose gel electrophoresis (DNA polymerase, dNTP, reaction buffer, glycerol, bromophenol blue, and stabilizer). PCR can be performed simply by adding primer pair and template.


  EzPCRTM BASIC 5x PCR Master Mix contains rTaq DNA polymerase that is a recombinant thermostable DNA polymerase from Thermus aquaticus. rTaq DNA polymerase has a 5’ à 3’ exonuclease activity and generates a 3’dA overhang. EzPCRTM BASIC 5x PCR Master Mix is recommended for use in conventional PCR process.

 

 

  As Master Mix is supplied as a 5x concentration format, users should adjust a final reaction to 1x (if final reaction volume is 20 ㎕, 4 ㎕ of 5x Master Mix should be added).

  One unit of rTaq DNA polymerase is contained in 4 ㎕ of 5x Master Mix.


 QC tests
  Performance test, exo/endo nuclease contamination test, stability test. genomic DNA contamination test.
 

Storage Condition

 

 

 

 

  Store at -20 ℃.

  EzPCRTM BASIC 5x PCR Master Mix is stable for at least 1years at recommended storage condition

 


Standard Protocol

1. Prepare the 20 ㎕ PCR solution as follows

 

     EzPCRTM BASIC 5x Master Mix

 4

    Primer (10 pmol/㎕) 

 each 0.5 

    Template DNA 

 0.1 - 50 ng

     PCR grade distilled water 

 -

     Add distilled water to make 20 ㎕ final volume.

 

 

2. Set PCR cycling as follows

 

  Initial denature at 95 ℃ : 3 min

 

 

 

< 1 kbp

 

 > 1 kbp

 

  Denature

 

95 ℃  

 

10 - 30 sec  

 

30 sec  

 

  Anneal

 

Tm ℃  

 

10 - 20 sec  

 

30 sec  

 

  Extend

 

72 ℃  

 

10 - 30 sec  

 

1 min / Kbp  

 

 

   * Set 25-35 PCR cycles for effective amplification.

 

 

 

 

 

 

 

 

 

 

FAQ
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