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EzPCR™ Basic 5x Master Mix (1 ml)
More Information
CAT #
EBT-7151
QTY
1ml (250 rxn / 4㎕)
PRICE (W)
50,000 원
MANUAL
MSDS
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Description

   EzPCR™ BASIC 5x PCR Master Mix is a ready-to-use premix containing all the components essential for a PCR and agarose gel electrophoresis (DNA polymerase, dNTP, reaction buffer, glycerol, bromophenol blue, and stabilizer). PCR can be performed simply by adding primer pair and template. EzPCR™ BASIC 5x PCR Master Mix contains rTaq DNA polymerase that is a recombinant thermostable DNA polymerase from Thermus aquaticus. rTaq DNA polymerase has a 5’ to 3’ exonuclease activity and generates a 3’dA overhang. EzPCRTM BASIC 5x PCR Master Mix is recommended for use in conventional PCR process. As Master Mix is supplied as a 5x concentration format, users should adjust a final reaction to 1x (if final reaction volume is 20 ㎕, 4 ㎕of 5x Master Mix should be added).

One unit of rTaq DNA polymerase is contained in 4 ㎕ of 5x Master Mix.


 QC tests
Performance test, exo/endo nuclease contamination test, stability test. genomic DNA contamination test.
 

Storage Condition

Store at -20 ℃.

EzPCR™ BASIC 5x PCR Master Mix is stable for at least 1years at recommended storage condition


Standard Protocol

1. Prepare the 20 ㎕ PCR solution as follows

 

    EzPCRTMBASIC 5x Master Mix

 4

    Primer (10 pmol/㎕) 

 each 0.5 

    Template DNA 

 0.1 - 50 ng

     PCR grade distilled water 

 -

     Add distilled water to make 20 ㎕ final volume.

 

 

2. Set PCR cycling as follows

 

  Initial denature at 95 ℃ : 3 min

 

 

 

< 1 kbp

 

 > 1 kbp

 

 Denature

 

95 ℃  

 

10 - 30 sec  

 

30 sec  

 

 Anneal

 

Tm ℃  

 

10 - 20 sec  

 

30 sec  

 

 Extend

 

72 ℃  

 

10 - 30 sec  

 

1 min / Kbp  

 

 

   * Set 25-35 PCR cycles for effective amplification.

 

 

 

 

 

 

 

 

 

 

FAQ
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