EzPCR™ Basic 5X Master Mix (5 ml)
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5 ml (1,250 rxn / 4 ㎕)
200,000 원
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  EzPCRTM BASIC 5X PCR Master Mix is a ready-to-use premix containing all the components essential for a PCR and agarose gel electrophoresis (DNA polymerase, dNTP, reaction buffer, glycerol, bromophenol blue, and stabilizer). PCR can be performed simply by adding primer pair and template.

  EzPCRTM BASIC 5X PCR Master Mix contains rTaq DNA polymerase that is a recombinant thermostable DNA polymerase from Thermus aquaticus. rTaq DNA polymerase has a 5’ à 3’ exonuclease activity and generates a 3’dA overhang. EzPCRTM BASIC 5X PCR Master Mix is recommended for use in conventional PCR process.



  As Master Mix is supplied as a 5X concentration format, users should adjust a final reaction to 1X (if final reaction volume is 20 ㎕, 4 ㎕of 5X Master Mix should be added).

  One unit of rTaq DNA polymerase is contained in 4 ㎕ of 5X Master Mix.

 QC tests
  Performance test, exo/endo nuclease contamination test, stability test. genomic DNA contamination test.

Storage Condition





  Store at -20 ℃.

  EzPCRTMBASIC 5X PCR Master Mix is stable for at least 1years at recommended storage condition


Standard Protocol

1. Prepare the 20 ㎕ PCR solution as follows


    EzPCRTMBASIC 5X Master Mix


    Primer (10 pmol/㎕) 

 each 0.5 

    Template DNA 

 0.1 - 50 ng

     PCR grade distilled water 


     Add distilled water to make 20 ㎕ final volume.



2. Set PCR cycling as follows


  Initial denature at 95 ℃ : 3 min




< 1 kbp


 > 1 kbp




95 ℃  


10 - 30 sec  


30 sec  




Tm ℃  


10 - 20 sec  


30 sec  




72 ℃  


10 - 30 sec  


1 min / Kbp  



   * Set 25-35 PCR cycles for effective amplification.











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