EzPCRTM BASIC Plus 2X PCR Premix is a ready-to-use premix containing all the components essential for a PCR and agarose gel electrophoresis (DNA polymerase, dNTP, reaction buffer, glycerol, bromophenol blue, and stabilizer). PCR can be performed simply by adding primer pair and template.

 

 

  EzPCRTM BASIC Plus 2X PCR Premix is amixtureof rTaq DNA polymerase and Pfu DNA Polymerase. rTaq DNA polymerase that is a recombinant thermostable DNA polymerase from Thermus aquaticus has a 5’ à 3’ exonuclease activity. EzPCRTM BASIC Plus 2X PCR Premix is recommended for use in conventional PCR process.

 

 

  As Master Mix is supplied as a 2X concentration format, users should adjust a final reaction to 1X (if final reaction volume is 20 ㎕, 10 ㎕of 2X Premix should be added).

  

 QC tests
  Performance test, exo/endo nuclease contamination test, stability test. genomic DNA contamination test.
 

Storage Condition

 

  Store at -20 ℃.

  EzPCR^TMBASIC Plus 2X PCR Premix is stable for at least 1 years at recommended storage condition

 

Standard Protocol

1. Prepare the 20 ㎕ PCR solution as follows

 

EzPCRTMBASIC Plus 2X Premix	10 ㎕
Primer (10 pmol/㎕)	each 0.5 ㎕
Template DNA	1 - 50 ng
PCR grade distilled water	-㎕
Add distilled water to make 20 ㎕ final volume.

 

 

2. Set PCR cycling as follows

 

Initial denature at 95 ℃ : 3 min
		< 1 kbp	> 1 kbp
Denature	95 ℃	10 - 20 sec	10 - 30 sec
Anneal	Tm ℃	10 - 20 sec	10 - 20 sec
Extend	72 ℃	10 - 30 sec	30 sec / Kbp

 

   * Set 25-35 PCR cycles for effective amplification.