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EzPCR™ Basic 2x Premix (96 tube)
More Information
CAT #
EBT-7122
QTY
96 tube (10 ㎕)
PRICE (W)
50,000 원
MANUAL
MSDS
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Description

 EzPCR™ BASIC 2x PCR Master Mix is a ready-to-use premix containing all the components essential for a PCR and agarose gel electrophoresis (DNA polymerase, dNTP, reaction buffer, glycerol, bromophenol blue, and stabilizer). PCR can be performed simply by adding primer pair and template. EzPCR™ BASIC 2x PCR Master Mix contains rTaq DNA polymerase that is a recombinant thermostable DNA polymerase from Thermus aquaticus. rTaq DNA polymerase has a 5’ to 3’ exonuclease activity and generates a 3’dA overhang. EzPCR™ BASIC 2x PCR Master Mix is recommended for use in conventional PCR process.

As Master Mix is supplied as a 2x concentration format, users should adjust a final reaction to 1x (if final reaction volume is 20 ㎕, 10 ㎕ of 2x Master Mix should be added).

One unit of rTaq DNA polymerase is contained in 10 ㎕ of 2x Master Mix.


QC tests

Performance test, exo/endo nuclease contamination test, stability test. genomic DNA contamination test.

Storage Condition

Store at -20 ℃.

EzPCR™ BASIC 2X PCR Master Mix is stable for at least 1years at recommended storage condition

 

Standard Protocol

1. Prepare the 20 ㎕ PCR solution as follows

 

    EzPCR™ BASIC 2x Master Mix

 10 ㎕

    Primer (10 pmol/㎕) 

 each 0.5 ㎕

    Template DNA 

 0.1 - 50 ng

     PCR grade distilled water 

 - ㎕

     Add distilled water to make 20 ㎕ final volume.

 

2. Set PCR cycling as follows

 

  Initial denature at 95 ℃ : 3 min 

 

 

< 1 kbp

 > 1 kbp

 Denature

95 ℃    

10 - 30 sec    

30 sec    

 Anneal

Tm ℃    

10 - 20 sec    

30 sec    

 Extend

72 ℃    

10 - 30 sec    

1 min / Kbp    

 

   * Set 25-35 PCR cycles for effective amplification.

FAQ
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