EzPCR™ Basic 5x Premix (480 tube)
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480 tube (4 ㎕)
240,000 원
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   EzPCR™ BASIC 5x PCR Master Mix is a ready-to-use premix containing all the components essential for a PCR and agarose gel electrophoresis (DNA polymerase, dNTP, reaction buffer, glycerol, bromophenol blue, and stabilizer). PCR can be performed simply by adding primer pair and template. EzPCR™ BASIC 5x PCR Master Mix contains rTaq DNA polymerase that is a recombinant thermostable DNA polymerase from Thermus aquaticus. rTaq DNA polymerase has a 5’ to 3’ exonuclease activity and generates a 3’dA overhang. EzPCR™ BASIC 5x PCR Master Mix is recommended for use in conventional PCR process. As Master Mix is supplied as a 5x concentration format, users should adjust a final reaction to 1x (if final reaction volume is 20 ㎕, 4 ㎕of 5x Master Mix should be added).

One unit of rTaq DNA polymerase is contained in 4 ㎕ of 5x Master Mix.

 QC tests
Performance test, exo/endo nuclease contamination test, stability test. genomic DNA contamination test.

Storage Condition

Store at -20 ℃.

EzPCR™ BASIC 5x PCR Master Mix is stable for at least 1years at recommended storage condition

Standard Protocol

1. Prepare the 20 ㎕ PCR solution as follows


    EzPCR™BASIC 5x Master Mix


    Primer (10 pmol/㎕) 

 each 0.5 

    Template DNA 

 0.1 - 50 ng

     PCR grade distilled water 


     Add distilled water to make 20 ㎕ final volume.



2. Set PCR cycling as follows


  Initial denature at 95 ℃ : 3 min




< 1 kbp


 > 1 kbp




95 ℃  


10 - 30 sec  


30 sec  




Tm ℃  


10 - 20 sec  


30 sec  




72 ℃  


10 - 30 sec  


1 min / Kbp  



   * Set 25-35 PCR cycles for effective amplification.

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