Description
EzPCR™ FAST
5X PCR Premix is a ready-to-use premix containing all the components essential
for a PCR and agarose gel electrophoresis (DNA polymerase, dNTP, reaction
buffer, glycerol, bromophenol blue, and stabilizer). PCR can be performed
simply by adding primer pair and template.
As Premix is supplied as a 5X concentration
format, users should adjust a final reaction to 1X (if final reaction volume is
20 ㎕ , 4 ㎕ of
5X Premix should be added). One unit of rTaq Plus
DNA Polymerase is contained in 4 ㎕ of
5X Premix.
rTaq
Plus DNA Polymerase is a thermostable DNA polymerase that designed for a
reliable amplification of long and complex targets with a robust yield in an
extremely short extension time. Due to its high elongation rate (about 100
bp/sec) and high processivity (about 250 bases), long sized products can be
generated within relatively shorter running time compared to any other
commercially available enzymes (5 kbp within 60 sec extension).
rTaq Plus DNA Polymerase has no
detectable exonuclease activity (5´-3´ or 3´-5´) and generates a 3´dA overhang,
suitable for a TA cloning of PCR products.
Applications
-
Fast PCR
-
RT-PCR
-
Amplification of cDNA and genomic DNA
-
Colony PCR
Qualifying Test
Performance test, exo/endo
nuclease contamination test, stability test. genomic DNA contamination test.
Storage Condition
Store at -20 ℃.
EzPCR™ LF 5X PCR Master Mix is stable for at least 1years at recommended storage condition.
Features
- 5’
→ 3’ exonuclease : no
- 3’ → 5’ exonuclease : no
- strand displacement : no
Storage
Condition
Store at -20 ℃ (stable up to 12
months).
Avoid
repeating freeze-thaw cycle
Standard
Protocol
